Abstract：OBJECTIVE To prepare the hyaluronic acid functionalized albumin nanoparticles with stable properties and low ocular irritation. METHODS Using coumarin 6 as fluorescent probe, the albumin nanoparticles were prepared by emulsification-sedimentation method, and hyaluronic acid was coupled to the surface of the nanoparticles in carbodiimide method. The substitution degree of hyaluronic acid was calculated with the reduction of amino groups which was determined by fluorescamine- derivatization method. The pharmaceutical properties and their ocular irritation to rabbits of nanoparticles were furtherly studied. RESULTS The degree of substitution of hyaluronic acid was 25.06% when functionlized by hyaluronic acid.The nanoparticles were spherical. The encapsulation rate is 86.94%, drug loading is 2.97%, and the cumulative release ratio was close to 70% in 48 h. Draize testing showed that the functionalized nanoparticles have no obvious irritation to rabbit eyes. CONCLUSION The hyaluronic acid functionalized albumin nanoparticles are successfully prepared. The degree of substitution of hyaluronic acid also can be measured. The obtained nanoparticles are less irritation. It is expected to use the nanoparticles as carriers to delivery drug to the back of the ocular.
任河, 李春玲, 宋璇, 秦迎丹, 孙玉琦. 透明质酸功能化白蛋白纳米粒的构建及眼刺激性评价[J]. 中国药学杂志, 2022, 57(14): 1173-1178.
REN He, LI Chun-ling, SONG Xuan, QIN Ying-dan, SUN Yu-qi. Preparation and Ocular Irritation Evaluation of Albumin Nanoparticles Functionalized by Hyaluronic Acid. Chinese Pharmaceutical Journal, 2022, 57(14): 1173-1178.
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