Determination of Multiple Components in Niuhuangshangqing Preparations by Chromatographic Peak Identification Assisted with Liner Calibration with Two Reference Substances
ZHANG Hong-wei1, ZHAO Yi-qing1, WANG Xiao-yan1, HUANG Xia1, SUN Lei2*, MA Shuang-cheng2*
1. Henan Institute for Food and Drug Control, NMPA Key Laboratory for Quality Control of Traditional Chinese Medicine(Chinese Materia Medica and Prepared Slices), Zhengzhou 450018, China; 2. National Instituts for Food and Drug Control, Beijing 100050, China
Abstract：OBJECTIVE To establish an analysis method for determination of four components in two kinds of Niuhuangshangqing preparations by HPLC and predicte the retention time by doable standard linear calibration method, in which the retention time was predicted by double standard linear calibration method and the prediction accuracy and column suitability were investigated in order to assist the chromatographic peak identification. METHODS The retention time of the four components in Niuhuangshangqing preparations were determined on 20 C18 columns of different brands and types.The standard retention time (ts) was calculated. Both geniposide (peak 1) and baicalin (peak 4) were taken as the linear calibration standards to forecast the retention time of the other two components. Other five C18 columns of different brands and types were used for validation. In comparison of liner calibration with the two reference substances,paeoniflorin a was used as the reference substance for the determination of relative correction factors of other three components. RESULTS Liner calibration with two reference substances was more accurate for predicting the retention time and suitable for more kinds of chromatographic columns than the relative retention time method. CONCLUSION The method of liner calibration with two reference substances can be used to assist the chromatographic peak identification, which is demonstrated to be practical and promising for multi-component assay of traditional Chinese medicine.
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ZHANG Hong-wei, ZHAO Yi-qing, WANG Xiao-yan, HUANG Xia, SUN Lei, MA Shuang-cheng. Determination of Multiple Components in Niuhuangshangqing Preparations by Chromatographic Peak Identification Assisted with Liner Calibration with Two Reference Substances. Chinese Pharmaceutical Journal, 2022, 57(5): 385-391.
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2022, Vol. 57 
