彝族药姜味草指纹图谱的建立和化学模式识别分析及含量测定

doi:10.11669/cpj.2022.05.003

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摘要目的建立彝族药姜味草的高效液相色谱法(HPLC)指纹图谱分析方法,结合化学模式识别,对其质量进行分析,并测定其中4种成分的含量。方法采用Agilent-C₁₈色谱柱(4.6 mm×250 mm,4 μm),以乙腈-0.2% 磷酸水为流动相,梯度洗脱,以迷迭香酸为参照,建立15批不同来源样品的HPLC特征图谱,结合相似度评价、聚类分析(HCA)、主成分分析(PCA)和正交偏最小二乘法-判别分析(OPLS-DA)等方法,进行药材质量评价,并测定4种成分的含量。结果建立了15批彝族药姜味草HPLC指纹图谱,标定20个共有峰,经与对照品比对指认出4个,其相似度都在0.960以上。通过HCA分析可将样品分为2类,结合 PCA和 OPLS-DA方法,发现其中5个影响质量的差异性组分。对15批样品中的咖啡酸、野黄芩苷、迷迭香酸和蒙花苷含量进行测定,显示4种成分在不同批次样品中的含量存在差异,且迷迭香酸和蒙花苷对区别不同来源样品贡献较大,与化学计量学方法分析结果一致。结论本研究所建立的HPLC指纹图谱方法简便、科学、稳定性好,为不同产地姜味草质量评价和定量分析提供了依据。

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关键词 ：姜味草, 高效液相色谱法, 指纹图谱, 主成分分析, 聚类分析, 正交偏最小二乘法-判别分析, 含量测定

Abstract：OBJECTIVE To establish the HPLC fingerprint of Yi Medicine Micromeria biflora (Buch.-Ham. ex D. Don) Benth, analyze its quality with chemical pattern recognition technology, and determine the contents of four components. METHODS The chromatographic analysis was conducted on Agilent-C₁₈ column (4.6 mm×250 mm, 4 μm). The mobile phase was acetonitrile-0.2% phosphoric acid eluted in gradient mode. Using rosmarinic acid as reference substance,the HPLC fingerprints of 15 batches of Yi Medicine Micromeria biflora (Buch.-Ham. ex D. Don) Benth from different sources were drawn. Then,further quality assessment of the drug was carried out by similarity evaluation, cluster analysis (CA), principal component analysis (PCA), and orthogonal partial least squares discriminant analysis (OPLS-DA). Meanwhile, the contents of four components were determined. RESULTS The fingerprints of 15 batches of Yi Medicine Micromeria biflora (Buch.-Ham. ex D. Don) Benth were established. There were 20 common peaks in the fingerprints and four common peaks were identified by reference substances. The fingerprint similarity of the samples was greater than 0.960. The samples were classified into two groups by hierarchical cluster analysis combined with principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA), and five components were the main markers that cause differences in the different batches of samples.The contents of trans-caffeic acid, scutellarin, rosmarinic acid and linarin in 15 batches of samples were determined. The results showed that the contents of four components in different batches of samples were different, in addition, rosmarinic acid and linarin contributed significantly to the difference of samples from different sources, which was consistent with the analysis results of chemometrics. CONCLUSION The HPLC fingerprint established in this paper is simple, scientific and stable, which can provide a basis for the quality evaluation and quantitative analysis of Yi Medicine Micromeria biflora (Buch.-Ham. ex D. Don) Benth in different producing areas.

Key words： Micromeria biflora (Buch.-Ham. ex D. Don) Benth. HPLC fingerprint principle component analysis cluster analysis orthogonal partial least squares discriminate analysis content determination

收稿日期: 2021-04-26

PACS:

R284

基金资助:中央引导地方科技发展资金项目资助(206Z2501G);河北省重点研发计划项目资助(20372502D);河北省自然科学基金项目资助(H2019423050);河北省中医药管理局项目资助(2022363)

通讯作者: ^*牛丽颖,女,教授,博士生导师研究方向:中药分析及药效物质基础研究 Tel:(0311)89926548

作者简介: 王梦,女,硕士研究生研究方向:中药质量控制

引用本文:

王梦, 田伟, 王鑫国, 甄亚钦, 支雅婧, 牛丽颖. 彝族药姜味草指纹图谱的建立和化学模式识别分析及含量测定[J]. 中国药学杂志, 2022, 57(5): 342-349. WANG Meng, TIAN Wei, WANG Xin-guo, ZHEN Ya-qin, ZHI Ya-jing, NIU Li-ying. Fingerprint Establishment, Chemistry Pattern Recognition Analysis and Content Determination of Multicomponent of Yi Medicine Micromeria biflora (Buch.-Ham. ex D. Don) Benth. Chinese Pharmaceutical Journal, 2022, 57(5): 342-349.

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[1]	LAN M. Herbs of South Yunnan(滇南本草) [M]. Vol 2. Kunming: Yunnan People's Publishing House, 1997:362.
[2]	Yunnan Food and Drug Administration. Standard of Chinese medicinal materials in Yunnan Province(云南省中药材标准) [M]. Vol 1. Kunming: Yunnan Science and Technology Press, 2005:358-366.
[3]	Chinese Materia Medica Editorial Board. Chinese Materia Medica(中华本草)[M]. Vol 19. Shanghai: Shanghai Science and Technology Press, 2000:6107.
[4]	HE R Y, WANG J Y, ZHANG W J, et al. Abstracts of original articles [J]. Chin Tradit Herb Drugs (中草药), 1993, 28(9): 536-537, 571.
[5]	ZHU G P. Study on chemical constituents of essential oil from Micromeria biflora [J]. Chin Tradit Herb Drugs (中草药), 1991, 22(3): 127.
[6]	WAN Q J, ZHANG Q Z, WANG W J, et al. Determination of oleanlic acid in Micromeria biflora from different districts of Yunnan province[J]. J Yunnan Univ Tradit Chin Med (云南中医学院学报), 2004, 27(4):10-11.
[7]	LI K P, PAN T L, ZHANG Y Y, et al. Studieson chemical constituents of Micromeria biflora benth. extractsby steam distillation and supercritical CO2 extraction [J]. J Guizhou Univ (Nat Sci)(贵州大学学报自然科学版), 2008, 25(2):165-168.
[8]	WAN Q J, ZHANG Q Z, ZHANG G F, et al. Study on chemical constituents of Micromeria biflora [J]. Chin Tradit Herb Drugs(中草药), 2005, 36(7):991-992.
[9]	LI K P, PAN T L, YANG X J, et al. Determination of ursolic acid and oleanolic acid in Micromeria biflora(Buch.-Ham.ex D.Don)Benth by RP-HPLC [J]. Chin Tradit Pat Med (中成药), 2009, 31(6):945-947.
[10]	CHENG S Y, FU Y, YU L N, et al. HPLC fingerprint and chemical pattern recognition of valeriana jatamansi [J]. Chin Pharm J (中国药学杂志), 2019, 54(6):489-493.
[11]	MENG Z, WANG L J, HUANG Y, et al. Analysis of fingerprint and pattern recognition of different parts of panax notoginseng [J]. Chin Pharm J (中国药学杂志), 2020, 55(7):504-509.
[12]	YAO C, XIN H, LU T L, et al.Comparing quality of gardenia in different places by HPLC[J]. Chin Pharm J (中国药学杂志), 2017, 52 (1):63-67.
[13]	WANG L L, SUN Q Y, YANG H H, et al. Pattern recognition and its application in quality assessment of Chinese material medica [ J]. Chin Tradit Herb Drugs(中草药), 2016, 47(23):4282-4288.
[14]	YUAN H J, LI W, ZHU Y F, et al. Study on fingerprint of Zhishi-Xiebai-Guizhi Decoction by HPLC and determination of 10 index components [J]. Chin Tradit Herb Drugs(中草药), 2020, 51(9): 2448-2459.
[15]	QUI J N, ZHANG Y, ZHANG S, et al. Quality control of Prunella vulgaris based on HPLC fingerprints combined with chemical pattern recognition and content determination [J]. Chin Tradit Herb Drugs(中草药), 2020, 51(10): 2842-2850.
[16]	LIANG J, WU W Y, SUN G X, et al. A dynamic multiple reaction monitoring method for the multiple components quantification of complex traditional Chinese medicine preparations: Niuhuang Shangqing pill as an example [J]. J Chromatogr A, 2013, 12(94):58-69.
[17]	PENG L, YANG B Y, ZHANG L, et al. HPLC fingerprint analysis and chemistry pattern recognition of wild and cultivated Polygalae Radix from different regions [J]. Chin Tradit Herb Drugs(中草药), 2018, 49(21):4998-5003.
[18]	ZHU S Q. Summary of research progress on the chemical constituents and pharmacology of perilla[J]. Biol Chem Eng (生物化工), 2018, 4(2): 148-149, 152.
[19]	CAO W, ZHANG W J, PAN J F, et al. Research progress of pharmacological action of rosmarinic acid [J]. Guangxi J Tradit Chin Med (广西中医药), 2019, 42(1):54-58.
[20]	ZHOU W, YANG H H, HUANG S H, et al. Antimicrobial and anti-lipid oxidation effects of Perilla leaf extract on the bonito fish fillet [J]. J Food Safe Qual (食品安全质量检测学报), 2017, 8(1): 41-49.
[21]	LI C M, CAI C C, ZHENG X J, et al. Orientin suppresses oxidized low-density lipoproteins induced inflammation and oxidative stress of macrophages in atherosclerosis[J]. Biosci Biotechnol Biochem, 2020, 84(4):774-779.
[22]	ZHANG L H, WU T T, ZHAO L G, et al. Advances in studies on anticancer activity of flavonoids from ginkgo biloba extract [J]. Chin Pharm J (中国药学杂志), 2019, 54(6):444-449.