HPLC同时测定通络化痰胶囊中14种成分的含量

doi:10.11669/cpj.2022.04.008

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摘要目的建立同时测定通络化痰胶囊中腺苷、没食子酸、天麻素、对羟基苯甲醇、丹酚酸B、牛磺熊去氧胆酸、人参皂苷Rb₁、牛磺鹅去氧胆酸、芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚和丹参酮Ⅱ_A14种成分的含量测定方法。方法以十八烷基键合硅胶为填充剂(Agilent Eclipse XDB C₁₈色谱柱),乙腈(A)-0.1%甲酸溶液(B)为流动相,梯度洗脱(0~20 min,5%A→25%A;20~40 min,25%A→40%A;40~60 min,40%A→85%A),柱温30 ℃,流速1 mL·min^-1,检测波长:220 nm,35~45 min,切换为203 nm。结果 14种成分可以实现完全分离,并与其他成分均能达到良好的分离;腺苷、没食子酸、天麻素、对羟基苯甲醇、丹酚酸B、牛磺熊去氧胆酸、人参皂苷Rb₁、牛磺鹅去氧胆酸、芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚和丹参酮Ⅱ_A分别在0.040 58～0.811 6、0.042 43～0.848 6、0.040 24～0.804 8、0.059 71～1.194、0.351 6～7.031、0.178 4～3.568、0.206 7～4.133、0.161 1～3.222、0.016 18～0.323 6、0.020 96～0.419 2、0.031 87～0.637 4、0.020 06～0.401 2、0.010 63～0.212 6、0.021 92～0.438 4 μg进样量范围内,与色谱峰峰面积呈良好线性响应;平均回收率(n=6)分别为98.2%,99.2%,99.1%,98.9%,99.3%,99.6%,98.9%,99.1%,98.9%,98.7%,99.1%,98.9%,98.7%和99.2%;RSD分别为1.0%,1.4%,1.3%,0.86%,0.84%,0.79%,1.2%,0.92%,1.1%,0.92%,0.69%,1.0%,0.93%和1.2%。结论该方法多种成分同时测定,操作简便,准确、重复性好,可用于通络化痰胶囊的质量控制。

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	林林
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关键词 ：高效液相色谱法, 通络化痰胶囊, 熊胆粉, 天麻, 三七, 丹参, 大黄, 多种成分同时测定

Abstract：OBJECTIVE To develop a method for determining 14 constituents in Tongluo Huatan capsules by HPLC. METHODS The quantitative analysis was carried out on Agilent Eclipse XDB C₁₈ column, using mobile phase consisting of acetonitrile (A)-0.1% formic acid solution (B) by a gradient elution program (0-20 min, 5%A→25%A; 20-40 min,25%A→40%A; 40-60 min,40%A→85%A) at a flow rate of 1 mL·min^-1 at 30 ℃. The detection wavelength was set at 220 nm, and changed to 203 nm between 35 and 45 min. RESULTS The linear ranges of adenosine, gallic acid, gastrodin, 4-hydroxybenzyl alcohol, salvianolic acid B, tauroursodeoxycholic acid,ginsenoside Rb₁, taurochenodeoxycholic acid,aloe-emodin, rhein, emodin, chrysophanol, physcion,and tanshinone Ⅱ_A fell within the ranges of 0.040 58-0.811 6, 0.042 43-0.848 6, 0.040 24-0.804 8, 0.059 71-1.194, 0.351 6-7.031, 0.178 4-3.568, 0.206 7-4.133, 0.161 1-3.222, 0.016 18-0.323 6, 0.020 96-0.419 2, 0.031 87-0.637 4, 0.020 06-0.401 2, 0.010 63-0.212 6 and 0.021 92-0.438 4 μg, respectively. The recoveries were 98.2%, 99.2%, 99.1%, 98.9%, 99.3%, 99.6%, 98.9%, 99.1%, 98.9%, 98.7%, 99.1%, 98.9%, 98.7% and 99.2%, respectively. The relative standard deviations were 1.0%, 1.4%, 1.3%, 0.86%, 0.84%, 0.79%, 1.2%, 0.92%, 1.1%, 0.92%, 0.69%, 1.0%, 0.93% and 1.2%, respectively (n=6). CONCLUSION This method is simple, accurate, reproducible and convenient for the quality control of Tongluo Huatan capsules.

Key words： HPLC Tongluo Huatan capsule bear bile powder Gastrodia elata Panax notoginseng Salvia miltiorrhiza Rheum officinale simultaneous differmination

收稿日期: 2021-06-30

PACS:

R917

基金资助:山东省自然科学基金项目资助(ZR2013HM074)

通讯作者: * 刘广桢,男,副主任药师研究方向:药品质量标准研究 Tel:(0531)81216521

作者简介: 林林,女,副主任药师研究方向:药品质量标准研究

引用本文:

林林, 谭会洁, 何慧, 刘广桢. HPLC同时测定通络化痰胶囊中14种成分的含量[J]. 中国药学杂志, 2022, 57(4): 295-300. LIN Lin, TAN Hui-jie, HE Hui, LIU Guang-zhen. Simultaneous Determination of Fourteen Constituents in Tongluo Huatan Capsules by HPLC. Chinese Pharmaceutical Journal, 2022, 57(4): 295-300.

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http://journal11.magtechjournal.com/Jwk_zgyxzz/CN/10.11669/cpj.2022.04.008 或 http://journal11.magtechjournal.com/Jwk_zgyxzz/CN/Y2022/V57/I4/295

[1]	YBZ00492009, National Medical Products Administration. Standards (Trial) (国家药品监督管理局标准(试行)YBZ00492009)[S]. 2009.
[2]	CHEN F, LONG H L, LEI H M, et al. Determination of tauroursodeoxycholic acid in Fufang Xiongdan capsules by HPLC [J]. China J Chin Mater Med(中国中药杂志), 2014, 39(5):838-840.
[3]	LI J J, DIAO F Y, HU D F, et al. Quality determination of seven effective components in Rhizoma Gastrodiae by HPLC [J]. J Pharm Res (药学研究), 2019, 38(4):205-208.
[4]	CHEN J P, ZHENG P, LI Z G, et al. Simultaneous determination of eight components in Jianpi Yishen pills by HPLC-MS [J]. Chin J Pharm Anal(药物分析杂志), 2017, 37(6):962-967.
[5]	ZHAI H Y, WANG H Y, YANG X L, et al. Study on HPLC specific chromatogram of Baoxinning tablets and simultaneous determination of its eigh index components [J]. Chin J Pharm Anal(药物分析杂志), 2017, 37(9):1633-1639.
[6]	HUA Z H, LIU D, L M C. Simultaneous determination of seven constituents in Ruangan Suopi pills by HPLC [J]. Chin J Pharm Anal(药物分析杂志), 2018, 38(4):703-709.
[7]	YANG G Y, XUE Z J, ZHANG D, et al. Chemical comparison of Salvia miltiorrhiza phloem and xylem based on fingerprint analysis and quantitative analysis of multi-components [J]. Chin Tradit Herb Drugs (中草药) . 2019, 50(21):5328-5335.
[8]	WEI J C, XIE Z, YANG Z T, et al. Simultaneous determination of gallic acid,cinnamic acid and catechin in 3 processed products of rheum officinaleby RP-HPLC [J]. J China Pharm(中国药房)2019, 30(22):3053-3056.
[9]	HUA Z H, LIU D, LI M C. Simultaneous determination of seven constituents in Ruangan Suopi pills by HPLC [J]. Chin J Pharm Anal(药物分析杂志), 2018, 38(4):703-709.
[10]	YE J, CHEN B, YU Y L. Simultaneous determination of eight compounds in Qingfei Yihuo tablets by HPLC [J]. Chin J Pharm Anal(药物分析杂志), 2017, 37(6):968-974.
[11]	GUO Q, WANG H B, LI X Y, et al. Simultaneous determination of 14 components in Lidanpaishi tablets by HPLC [J]. Chin J Pharm Anal(药物分析杂志), 2017, 37(4):631-638.
[12]	DOU Z H, QIAO J, BIAN L, et al. Combinational quality control method of rhei radix et rhizoma based on fingerprint and QAMS [J]. Chin Pharm J (中国药学杂志), 2015, 50(5):442-448.
[13]	LIN L, LIU G Z, YU F R, et al. Simultaneous determination of twelve constituents in Yigan Jiedu Capsules by HPLC [J]. Chin Phar J (中国药学杂志), 2020, 55(7):557-561.