DPPH Kinetic Characteristics and Identification of Antioxidant Compounds in Eucommia ulmoides Leaves
TU Wan-qian1, ZHANG Liu-ji1*, XIA Man-yu2, LI Ning2, LI Xiang-yang3
1. Henan Engineering Center of Genuine Medicinal Materials Comprehensive Development, Henan Academy of Chinese Medicine, Zhengzhou 450004, China; 2. Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China; 3. Henan Institute for Food and Drug Control, Zhengzhou 450018, China
Abstract：OBJECTIVE To study the dynamic characteristics of DPPH scavenging activity of Eucommia ulmoides leaves, and identify the antioxidant active compounds. METHODS The influence of different concentrations of E. ulmoides leaves, reaction temperature and reaction time were compared. IC50 of DPPH free radical scavenging rate was determined. DPPH-HPLC was used to analyze the content changes of the main compounds in E. ulmoides leaves before and after reaction with DPPH. RESULTS The concentrations of E. ulmoides leaves, reaction temperature and reaction time could affect DPPH free radical scavenging rate. The DPPH free radical scavenging rate of E. ulmoides leaves increased with the raise of concentration E. ulmoides leaves, reaction temperature and reaction time correspondingly. The IC50 of DPPH free radical scavenging rate was 0.509 mg·mL-1. Compared with the contents before reaction with DPPH, the contents of aucubin, geniposidic acid in E. ulmoides leaves decreased little; while the contents of chlorogenic acid, rutin, isoquercitrin and astragalin decreased significantly. It suggested that chlorogenic acid, rutin, isoquercitrin and astragalin might be main antioxidant compounds in E. ulmoides leaves. CONCLUSION The dynamic characteristics of DPPH free radical scaveging activity of E. ulmoides leaves are preliminarily known. The antioxidant compounds in Eucommia ulmoides leaves were identified, which could provide reference for quality control of E. ulmoides leaves related to antioxidant activity.
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2022, Vol. 57 
