Total Flavonoids of Anchusa italica Retz. Inhibit Activation of Inflammasome through ROS/TXNIP/NLRP3 Pathway to Protect against Myocardial Ischemia-reperfusion Injury
YIN Su-yue, JIANG Yu, WANG Dan-shu, YAN Liu-yan, WANG Shou-bao*, DU Guan-hua*
Beijing Key Lab of Drug Target Identification and Drug Screening, Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100050, China
Abstract：OBJECTIVE To investigate the role of activation of NLRP3 inflammasome and ROS/TXNIP/NLRP3 pathway in the effects of total flavonoids of Anchusa italica Retz. (TF) against myocardial ischemia-reperfusion injury (MIRI). METHODS The ligation of left coronary artery was used to mimic MIRI in mice. Thirty mice were randomly divided into I/R group, 30 mg·kg-1 TF treatment group (I/R+TF30) and 100 mg·kg-1 treatment group (I/R+TF100). At 24 h after reperfusion, cardiac function, myocardial infarct index and serum myocardial enzyme were measured. The activation of NLRP3 inflammasome, ROS level and TXNIP/NLRP3 interaction were observed. RESULTS TF treatment at the doses of 30 and 100 mg·kg-1 improved cardiac function, reduced myocardial infarct index, showing obvious myocardial protection. In addition, TF treatment reduced the levels of inflammatory cytokines IL-1 β, IL-6 and TNF-α, and decreased ROS content in myocardial tissue. The levels of TXNIP, NLRP3, cleaved caspase-1 and cleaved IL-1 β were significantly decreased in I/R+TF30 and I/R+TF100 groups, and the TXNIP/NLRP3 interaction was also inhibited as compared with I/R group. CONCLUSION TF treatment exertes potent protection against MIRI in mice, in which inhibition of activation of NLRP3 inflammasome via the ROS/TXNI/NLRP3 pathway played a pivotal role.
阴苏月, 姜瑜, 王丹姝, 燕柳艳, 王守宝, 杜冠华. 牛舌草总黄酮通过ROS/TXNIP/NLRP3抑制炎症小体活化抗心肌缺血再灌注损伤[J]. 中国药学杂志, 2021, 56(14): 1131-1137.
YIN Su-yue, JIANG Yu, WANG Dan-shu, YAN Liu-yan, WANG Shou-bao, DU Guan-hua. Total Flavonoids of Anchusa italica Retz. Inhibit Activation of Inflammasome through ROS/TXNIP/NLRP3 Pathway to Protect against Myocardial Ischemia-reperfusion Injury. Chinese Pharmaceutical Journal, 2021, 56(14): 1131-1137.
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2021, Vol. 56 
