Chemical Constituents from the Stems and Leaves of Dioscorea opposite Thunb.
REN Ying-jie, CAO Yan-gang, ZENG Meng-nan, ZHANG Bei-bei, LIU Yan-ling, WANG Meng-na, HE Chen, ZHANG Yan-li, ZHENG Xiao-ke, FENG Wei-sheng*
Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment and Chinese Medicine Development of Henan Province, Henan University of Chinese Medicine, Zhengzhou 450046, China
Abstract：OBJECTIVE To investigate the chemical constituents from the dried stems and leaves of Dioscotea opposita Thunb. METHODS The compounds were isolated and purified by silica gel, Sephadex LH-20, and semi-preparative HPLC, and their structures were elucidated based on NMR and MS data. The anticancer activities of isolated compounds were evaluated using a decrease proliferation assay in HepG2 cells. RESULTS Twenty-six compounds were isolated from the 50% acetone extract of the dried stems and leaves of D. opposita Thunb., which were identified as apigenin (1), vitexin (2), chrysoeriol(3), isovitexin(4), 8-C-rhamnosylapigenin(5), chrysoeriol-7-O-β-D-glucoside (6), 3′-O-methylepicatechin-7-O-β-D-glucopyranoside(7), chrysoeriol-7-O-sophoroside(8), drymariatin C (9), secoisolariciresinol(10), benzyl-β-D-glucoside(11), northalifoline (12), paeoniflorin (13), euscaphic acid (14), breyniaionoside D(15), 4-hydroxy-3,5-dimethoxybenzaldehyde (16), 1, 3, 5-trimethoxybenzene (17), 3-methoxy-4-hydroxyphenylethanol(18), 3, 4′-dihydroxy-3′-methoxypropiophenone (19), 4-hydroxybenzoic acid (20), 4-hydroxybenzaldehyde (21), vanillic acid (22), ferulic acid (23), trans-cinnamic acid (24), benzoic acid (25), and coumaric acid (26). CONCLUSION Twenty-one compounds including compounds 2, 4-10, 12-17, 19-20, 22-26 are isolated from this plant for the first time, 17 compounds including compounds 2, 4-10, 12-15, 17, 19, 23-25 are isolated from the genus Dioscorea L. for the first time, and compounds 1, 4, 8, 12-14, 18, 20 could decrease proliferation of HepG2 cells at the dose of 10 μmol·L-1, indicating that the compounds have anticancer activity.
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2021, Vol. 56 
