目的 采用正交设计结合光学相干断层扫描法(OCT)优选桂皮醛脂质体透皮吸收的最佳微针条件,为桂皮醛作为经皮制剂治疗皮肤光老化提供参考。方法 采用正交设计,以12 h内累积经皮渗透量和60 min内皮肤恢复程度为指标,考察微针长度,作用时间和作用力大小对桂皮醛脂质体的促透作用,选择最佳微针条件。结果 最终筛选出的最佳微针条件为250 μm,5 N,2 min,该条件下皮肤的累积透过量较高,并且在60 min内的皮肤恢复程度最强,2个指标的综合评分最高。结论 该条件是在综合考虑微针作用的有效性和安全性基础上筛选出的,具有一定可信性,也可为多种方式的联合促渗提供参考。
Abstract
OBJECTIVE To optimize the best microneedle condition for transdermal absorption of cinnamaldehyde liposomes using orthogonal experiments combined with optical coherence tomography (OCT), and then provide a reference for cinnamaldehyde as a transdermal preparation for skin photoaging. METHODS The orthogonal experiment was carried out with cumulative transdermal penetration in 12 h and the recovery of skin within 1 h as indicators, the effects of microneedle length, action time and force were investigated on the permeability of cinnamaldehyde liposomes,and then the optimal microneedle conditions was selected. RESULTS The optimal microneedle condition was 250 μm, 5 N, 2 min. Under this condition, the cumulative penetration of the skin was higher, and the skin recovery degree was the strongest within 60 min. The comprehensive score of the two indicators was the highest. CONCLUSION This condition is credible and can make a reference for multiple ways of joint penetration promotion for which was selected on the basis of comprehensive consideration of the effectiveness and safety of microneedle.
关键词
桂皮醛脂质体 /
累积经皮渗透量 /
光学相干断层扫描系统 /
微针 /
正交设计
{{custom_keyword}} /
Key words
cinnamaldehyde liposome /
cumulative transdermal penetration /
OCT system /
microneedle /
orthogonal design
{{custom_keyword}} /
中图分类号：
R944
{{custom_clc.code}}
({{custom_clc.text}})
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] ZHOU L, YAN H B. Effect of cinnamic aldehyde on expression of MMP-1, MMP-3 and MAPK signal in human dermal fibroblasts after UVA irradiation[J]. Chin J Dermatovenereol(中国皮肤性病学志), 2015,(2):114-117.
[2] GAO Y,ZHENG M C,DU S Y,et al. Preparation technology of cinnamaldehyde liposomes [J]. J Tianjin Univ Tradit Chin Med(天津中医药大学学报),2019,38(2):180-185.
[3] XIA X,GAO W Y,LIU X Y,et al. Effect of metallic arraymicroneedle pretreatment on ramosetron hydrochloride paches in transdermal drug delivery [J]. Chin Pharm J(中国药学杂志), 2019,54(2):105-109.
[4] MENG L J,QIAO J X,LIN P T,et al. Microneedle percutaneous drug delivery system, wound repair and pharmacology evaluation [J]. Chin Pharm J(中国药学杂志),2017,52(6):434-437.
[5] GUO T, LU J Y, ZHAO J H,et al. Effects of microneedle combining nanostructured lipid carriers in transdermal delivery for Aconitum sinomontanum [J]. Chin J Tradit Chin Med Pharm(中华中医药杂志),2018,33(5):432-436.
[6] ZHAN H H,HUANG Y C,MA F S. Research progress in the evaluation methods of microneedles puncture performance [J]. Chin Pharm J(中国药学杂志),2018,53(22):1890-1895.
[7] LI Z, SONG Y L, LIN J, et al. The application progress of optical coherence tomography in the evaluation of the atherosclerotic plaques [J]. Chin J Stroke (中国卒中杂志),2018,13(8):877-880.
[8] CUI Y H, MO Y J, ZHANG Q, et al. Microneedle-assisted percutaneous delivery of paeoniflorin-loaded ethosomes [J]. Molecules,2018, 23(12):115-130.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}
PDF(1769 KB)
