Exploration of Mechanism of the Analgesic Effect of Amomi Fructus Based on Network Pharmacology
REN Juan1, ZHANG Na1, WANG Min1, LIAO Jia-bao2, ZHANG Li-ju1*, WANG Ze1, CUI Guo-zhen1, SUN Zhi-yong1*
1. Department of Bioengineering, Zunyi Medical University at Zhuhai Campus, Zhuhai 519041, China; 2. China Resources Sanjiu Medical & Pharmaceutical Co., Ltd., Shenzhen 518110, China
Abstract：OBJECTIVE To explore the pharmacological mechanism of traditional Chinese medicine (TCM) Amomi Fructus by network pharmacology. METHODS The analgesic effect of Amomi Fructus was firstly investigated by in vitro experiment. Then, a compound database of Amomi Fructus was established by analyzing related literatures and network pharmacology method. At last, molecular docking techniques were performed to evaluate the interaction of potential active ingredients of Amomi Fructus with predicted protein targets. RESULTS It was found that the active constituents of Amomi Fructus had inhibitory effects on cyclooxygenase-2 activity in the range of 0-100 μg·mL-1, and the IC50 was measured to be 19.20 μg·mL-1, indicating its potential analgesic effect. By TCMSP database and CNKI database analysis, a total number of 18 potential active ingredients and 60 predicted protein targets were obtained. CONCLUSION The research confirms the analgesic effect of Amomi Fructus by in vitro experiment. It is discovered that the analgesic effect of Amomi Fructus is possibly involved with the interaction with gamma-aminobutyric-acid receptor, prostaglandin G/H synthase, and sodium channel proteins. This study provides a new perspective for the study of the analgesic mechanism of Amomi Fructus.
任娟, 张娜, 王敏, 廖嘉宝, 张丽菊, 汪泽, 崔国祯, 孙智勇. 基于网络药理学的砂仁镇痛作用机制研究[J]. 中国药学杂志, 2021, 56(9): 723-730.
REN Juan, ZHANG Na, WANG Min, LIAO Jia-bao, ZHANG Li-ju, WANG Ze, CUI Guo-zhen, SUN Zhi-yong. Exploration of Mechanism of the Analgesic Effect of Amomi Fructus Based on Network Pharmacology. Chinese Pharmaceutical Journal, 2021, 56(9): 723-730.
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