Determination of the Compounds of Dendrobium pendulum Roxb. by UPLC-Q-TOF-MS
WANG Yuan-cheng1, HAN Bin2, LI Zhen-jian1*, NIE Xue-ting1, YANG Ming-zhi3, WANG Wei1, SUN Zhen-yuan1
1. Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China;
2. Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;
3. Dendrobium Professional Committee of China Association of Traditional Chinese Medicine, Kunming 650002, China
OBJECTIVE To study the stems, leaves and flowers from Dendrobium pendulum Roxb qualitatively and quantitatively by UPLC-Q-TOF-MS. METHODS Formic acid and acetonitrile were used as mobile phase and gradient elution was conducted. ESI was used, and mass scanning was performed in the range of m/z 50-1 000. RESULTS Thirteen components were isolated. They were identified as dendrocandin L (1), rutin (2), hyperoside (3), densiflorol A (4), tristin (5), apigenin (6), naringenin (7), cannabistilbene Ⅱ(8), longicornuol A (9), aloifol I (10), coelonin (11), isomoniliformine A (12), denchryside A (13), including bibenzyls, flavonoids, phenanthrene, biphenanthrene, etc. The contents of bibenzyls in the flowers of D. pendulum were higher than those in the stems and leaves, and the contents of flavonoids in the leaves were higher with rutin of 23.498 μg·g-1. CONCLUSION Cannabistilbene Ⅱ is isolated from Orchidaceae for the first time, and 12 components are obtained in D. pendulum for the first time.
王元成, 韩彬, 李振坚, 聂雪婷, 杨明志, 王伟, 孙振元. 基于UPLC-Q-TOF-MS的肿节石斛化学成分研究[J]. 中国药学杂志, 2021, 56(9): 708-714.
WANG Yuan-cheng, HAN Bin, LI Zhen-jian, NIE Xue-ting, YANG Ming-zhi, WANG Wei, SUN Zhen-yuan. Determination of the Compounds of Dendrobium pendulum Roxb. by UPLC-Q-TOF-MS. Chinese Pharmaceutical Journal, 2021, 56(9): 708-714.
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2021, Vol. 56 
