Abstract：OBJECTIVE To select the best extraction method for total flavonoids in Cuscuta chinensis and determine the estrogenic activity of the extract. METHODS A method for the content determination of total flavonoids in Cuscuta chinensis was established by UV spectrophotometry. The orthogonal design L4(23) was used to optimize and screen the ultrasonic extraction, reflux extraction and flash extraction with the content of total flavonoids as the evaluation index. The uterine coefficient and endometrial thickness were used to evaluate the estrogenic activity of Cuscuta chinensis in immature mice. RESULTS The flash extraction method showed the shortest time and highest total flavonoids content among the three extraction methods. The extraction process was as follows: 70% ethanol as the extraction solvent, the ratio of material to liquid of 1∶40, the cumulative extraction time of 2 minutes and the extraction voltage of 100 V. The results of estrogen activity study showed that the total flavonoids in Cuscuta chinensis could significantly increase uterine weight and endometrial thickness in immature mice. CONCLUSION The flash extraction method is energy efficient and suitable for the extraction of total flavonoids in Cuscuta chinensis. The total flavonoids in Cuscuta chinensis has good estrogenic activity, which can provide a reference for the rational development and utilization of Cuscuta chinensis functional products.
张馨元, 孙向明, 宋辉, 徐倩, 王美尧. 菟丝子总黄酮的提取方法筛选及其雌激素活性研究[J]. 中国药学杂志, 2021, 56(1): 23-28.
ZHANG Xin-yuan, SUN Xiang-ming, SONG Hui, XU Qian, WANG Mei-yao. Screening of Extraction Method for Total Flavonoids in Cuscuta chinensis and Study on its Estrogenic Activity. Chinese Pharmaceutical Journal, 2021, 56(1): 23-28.
DONNAPEE S, LI J, YANG X, et al. Cuscuta chinensis Lam.: a systematic review on ethnopharmacology, phytochemistry and pharmacology of an important traditional herbal medicine[J]. J Ethnopharmacol, 2014, 157: 292-308.
[3]
LIN M K, LEE M S, HUANG H C, et al. Cuscuta chinensis and C. campestris attenuate scopolamine-induced memory deficit and oxidative damage in mice[J]. Molecules, 2018, 23(12): E3060.
[4]
SUN S L, GUO L, REN Y C, et al. Anti-apoptosis effect of polysaccharide isolated from the seeds of Cuscuta chinensis Lam on cardiomyocytes in aging rats[J]. Mol Biol Rep, 2014, 41(9): 6117-6124.
[5]
KANG S Y, JUNG H W, LEE M Y, et al. Effect of the semen extract of Cuscuta chinensis on inflammatory responses in LPS-stimulated BV-2 microglia[J]. Chin J Nat Med(中国天然药物), 2014,12(8): 573-581.
[6]
KIM H J, YANG H, JUNG D H, et al. Ameliorating effects of Cuscuta chinensis Lamak extract on hindlimb ischemia, and angiogenic or inflammatory associated factors in ovariectomized mice [J]. Mol Med Rep, 2019, 19(4): 3321-3329.
[7]
MIAO M S, PENG M F, YAN X L. Effect of dodder total flavone on polycystic ovary syndrome rat models [J]. Chin J Exp Tradit Med Form (中国实验方剂学杂志), 2019, 29(5): 143-150.
[8]
GUO C, WANG Y J, ZHANG J P. Studies on chemical constituents and pharmacological activities of Cuscuta chinensis[J]. Mod Med Health, 2005, 16(10): 1035-1036.
[9]
ZHAO P W, WANG D W, NIU J Z, et al. Evaluation on phytoestrogen effects of ten kinds of Chinese medicine including flos carthami [J]. China J Chin Mater Med (中国中药杂志), 2007, 32(5): 436-439.
[10]
LI W L, DING J X, ZANG B S, et al. Analysis of purified active ingredients of estrogen mimic effect in Cuscuta chinensis by HPLC-Q TOF-MS/MS[J]. Chin Pharm J (中国药学杂志), 2014, 49(20): 1791-1795.
[11]
DING J X, LI W L, HU Y, et al. Characterization of estrogenic active ingredients in Cuscuta chinensis Lam. based on spectral characteristics and high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry[J]. Mol Med Rep, 2019, 19 (2): 1238-1247.
[12]
GUO C, ZHANG Z Y, ZHENG H C, et al. The characterstics of 1H-NMR and 13C-NMR spectra of BBI alkaloids [J]. China J Chin Mater Med (中国中药杂志), 1990, 15(3): 138-140.
[13]
QU M, TANG Q S. Optimization of extraction technology of Shenqijieyu prescription with orthogonal test [J]. World Sc Technol/Modernization Tradit Chin Med Mater Med(世界科学技术-中医药现代化), 2018, 20(6): 917-921.
[14]
WANG H Y, SHA Y Z, LI D M, et al. Comparative study on microwave-assisted and ultrasonic-assisted extraction of peach kernel oil[J]. Chem Ind Forest Prod(林产化学与工业), 2019, 39(6): 44-52.
[15]
YANG T M, LI C, FU H Y, et al. Study on flash extracyion process of total flavonoids from Cuscuta chinensis and its content determiation by near infrared transmittance spectroscopy [J]. Chem Bioeng(化学与生物工程), 2012, 29(9): 87-90.
[16]
LI W L, ZHANG Y, BAI J, et al. Identification of chemical constituents in Cuscuta chinensis using HPLC-ESI/Q-TOF MS/MS[J]. Bio Technol Indian J, 2013, 8(4): 563-567.
[17]
ZENG Y L, CHEN X N, TAN J W, et al. Application of smashing tissue extraction process to the technology of Chinese drugs preparation[J]. Western J Tradit Chin Med(西部中医药), 2018, 31(2): 26-29.
[18]
LI J Y, WANG L Z, YAN G L, et al. Study on extraction process of total flavonoids in dodder [J]. J Harbin Univ Commer(Nat Sci Ed)(哈尔滨商业大学学报:自然科学版), 2011, 27(1): 5-6,11.
[19]
GNAN Q X, YANG J,HUANG Q J, et al. Research process of new extraction technology for salvia [J]. J Harbin Univ Commer(Nat Sci Ed)(哈尔滨商业大学学报:自然科学版), 2013, 29(3): 262-264,270.
[20]
SHELBY M D, NEWBOLD R R, TULLY D B, et al. Assessing environmental chemicals for estrogenicity using a combination of in vitro and in vivo assays[J]. Environ Health Perspec, 1996, 104(12): 1296-1300.
[21]
COLBORN T, VOM SAAL F S, SOTO A M. Developmental effects of endocrine-disruption chemical[J]. Environ Health Perspec, 1993, 101(2): 386-387.
2021, Vol. 56 
