Abstract：OBJECTIVE To develop a method for simultaneous determination of flavanols, isoflavones, flavonols, dihydroflavones, flavanonols, chalcones, pterocarpan, anthocyanidins and phenolic acids in Spatholobi Caulis from different habitats and commercial herbs by UFLC-QTRAP-MS/MS. Principal component analysis (PCA) was applied to comprehensively analyze and evaluate the results of multiple active constituents. METHODS The chromatographic separation was performed on an XBridge C18 column (4.6 mm×100 mm, 3.5 μm) at 30 ℃ with gradient elution by 0.3% formic acid aqueous solution-methanol, and the flow rate was 0.8 mL·min-1, using multiple-reaction monitoring (MRM) mode. Principal component analysis (PCA) was performed to evaluate 31 samples according to the contents of 22 constituents. RESULTS The 22 target components showed good linearity (r>0.999 0) in the range of the tested concentrations. The average recovery rates of the 22 components were from 97.46% to 103.6% with relative standard deviations less than 5%. CONCLUSION The developed method is accurate and reliable, and could be used for simultaneous determination of multi-functional substances or index components in Spatholobi Caulis, which could provide a reference for the comprehensive evaluation and quality control of Spatholobi Caulis.
梅余琪, 魏丽芳, 邹立思, 刘训红, 陈佳丽, 谈梦霞, 王程成, 蔡芷辰, 张芙蓉. 超快速液相色谱-三重四级杆/线性离子阱质谱法同时测定鸡血藤不同产地及商品药材中多元活性成分[J]. 中国药学杂志, 2020, 55(24): 2012-2020.
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2020, Vol. 55 
