Inhibitory Effect of Cardamonin on Human Osteosarcoma Cells and Its Underlying Mechanism
ZHANG Lu-lu1, YANG Chun-mei1, HUANG Yan-ran2, HUANG Hua-kun1, YUAN Xiao-hui1, ZHANG Ping1, YE Cai-hong1, WEI Meng-qi1, LUO Xiao-ji2, LUO Jin-yong1*
1. College of Medical Laboratory Science, Chongqing Medical University, Key Laboratory of Clinical Laboratory Diagnostics, Ministry of Education, Chongqing 400016, China; 2. Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
Abstract：OBJECTIVE To analyze and confirm the inhibitory effect of cardamonin(CAR) on human osteosarcoma(OS) cells and its related mechanism. METHODS Human osteosarcoma cells were treated with 0, 4, 12, 16 μmol·L-1 CAR and 0.08% DMSO, respectively. Cell proliferation was detected by crystal violet staining and MTT assay. Cell apoptosis was detected by Hoechst staining and flow cytometry(FCM). Cell migration ability was detected by scratch healing test. Cell invasion ability was detected by Transwell method. Western blot detects changes in cell proliferation, apoptosis, migration and invasion-associated proteins and Wnt/β-catenin signaling pathway-related proteins. RESULTS CAR inhibits proliferation, migration and invasion of osteosarcoma cells. CAR inhibits the expression of PCNA, MMP-7 and vimentin. CAR inhibits the expression of Bcl-2, promotes the expression of apoptotic markers caspase 3 and cleaved-caspase 3. CAR inhibits the expression of the key molecule β-catenin of Wnt/β-catenin signaling and its downstream target molecules cyclin D1 and c-Myc. CONCLUSION CAR can inhibit the proliferation, invasion and migration of osteosarcoma cells, but promote its apoptosis. Its molecular mechanism may be related to the interference of Wnt/beta-catenin signaling pathway activation.
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2020, Vol. 55 
