Construction of Matrine Solid Lipid Nanoparticle and Determination of the Entrapment Efficiency
JIN Wen-zhen, TAN Hua-jin, WANG Kai-kang, TAN Xiao-chuan, ZHANG Yu-jia, ZHENG Wen-sheng*
Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing City Key Laboratory of Drug Delivery Technology and Novel Formulation, Beijing 100050, China
Abstract：OBJECTIVE To prepare matrine solid lipoid nanoparticle,establish preparating method and determine the encapshlation efficiency. METHODS Matrine solid lipoid nanoparticle was prepared by microemulsion-probe ultrasonic method and its quality was evaluated by particle size, Zeta potential, microscopic morphology and in vitro release. The encapsulation efficiency of the carrier was measured by different methods and their effect was compared. RESULTS The diameter of matrine solid lipoid nanoparticle was (116.7±2.6) nm and its Zeta potential was (-45±1.7)mV. Transmission electron micrographs showed that the solid lipoid nanoparticle was uniform in size and spherical. The in vitro release result suggested the carrier exhibited control release character. Dextran gel microcolumn centrifugation can effectively separate free drugs and carriers, and the measured encapsulation efficiency data has little difference in stability. CONCLUSION Matrine solid lipoid nanoparticle is successfully prepared and their particle size, Zeta potential and in vitro release quality are evaluated.Dextran gel microcolumn method is effective in the measurement of matrine solid lipoid nanoparticle, providing a reliable reference for the determination of water-soluble drug encapsulation efficiency.
靳雯臻, 檀华进, 汪凯康, 谭晓川, 张宇佳, 郑稳生. 苦参碱固体脂质纳米粒的构建及包封率测定方法研究[J]. 中国药学杂志, 2020, 55(12): 1015-1020.
JIN Wen-zhen, TAN Hua-jin, WANG Kai-kang, TAN Xiao-chuan, ZHANG Yu-jia, ZHENG Wen-sheng. Construction of Matrine Solid Lipid Nanoparticle and Determination of the Entrapment Efficiency. Chinese Pharmaceutical Journal, 2020, 55(12): 1015-1020.
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2020, Vol. 55 
