贵州医科大学, a. 贵州省药物制剂重点实验室,省部共建药用植物功效与利用国家重点实验室;b. 民族药与中药开发应用教育部工程研究中心;c. 药学院, 贵阳 550004
Investigation of the Excretion of Seven Constituents of Inula cappaort in Rat Urine by UPLC-MS/MS
LI Naa,c, YANG Shu-tinga,c, LU Yuana, GONG Zi-penga, LI Yong-junb, CAO Chuangc, WANG Yong-lina*
a. State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Provincial Key Laboratory of Pharmaceutics;b. Engineering Research Center for the Development and Application of Ethnic Medicine and TCM Ministry of Education;c. School of Pharmacy, Guizhou Medical University, Guiyang 550004, China
Abstract：OBJECTIVE To establish an UPLC-MS/MS method for the analysis of seven compounds of Inula cappaort in rat urine to study their excretion. METHODS The urine samples in 0-2, 2-6, 6-12, 12-24, and 24-36 h were collected. Acquity UPLC BEH C18 column (2.1 mm×50 mm, 1.7 μm) was used and the column temperature was set at 45 ℃, the mobile phase was 0.1% formic acid acetonitrile -0.1% formic acid aqueous solution in a gradient elution mode and flow rate was 0.25 mL·min-1. The detection was carried out by a triple quadrupole linear ion trap mass spectrometer in positive and negative ion mode with an electrospray source. Multiple reactions monitoring (MRM) mode was employed. RESULTS The calibration curves showed good linearity, with correlation coefficients of greater than 0.991 0 for all of the analytes within the concentration ranges. The intra-day and inter-day precisions (RSD) were all less than 15%. The extraction recoveries of the seven components were more than 84.41%, without obvious matrix effect, which met the requirements for analysis. CONCLUSION The established method is simple, rapid, and sensitive. It can be applied in the excretion study of the seven components of Inula cappaort extract in rat urine. The urine excretion test showed that the prototype excretion rates are low in rats, and the cumulative excretion rates are all less than 5%.
黎娜, 杨淑婷, 陆苑, 巩仔鹏, 李勇军, 曹闯, 王永林. 利用超高效液相色谱质谱联用分析羊耳菊7种成分在大鼠尿液中的排泄过程[J]. 中国药学杂志, 2019, 54(21): 1797-1803.
LI Na, YANG Shu-ting, LU Yuan, GONG Zi-peng, LI Yong-jun, CAO Chuang, WANG Yong-lin. Investigation of the Excretion of Seven Constituents of Inula cappaort in Rat Urine by UPLC-MS/MS. Chinese Pharmaceutical Journal, 2019, 54(21): 1797-1803.
GONG Z P, XIONG D F F, LI M, et al. Analysis of chemical constituents of effective components of Inula cappa[J]. J Anhui Agri Sci (安徽农业科学),2017,45(29):131-133+152.
WANG A M, LI M, SUN J, et al. Study on antibacterial and anti-inflammatory activities of Inula cappa[J]. Lishizhen Med Mater Med Res(时珍国医国药), 2018,29(7):1580-1584.
GONG Z P, LI M, XIONG D F F, et al. Evaluation of anti-inflammatory activity of Inula cappa extract based on inflammation model in vitro[J]. Nat Prod Res Dev(天然产物研究与开发),2017,29(12):2050-2055.
MO J J, XU M D, YANG D D, et al. Experimental study on anti-inflam matory and analgesic efects of ethanol extracts of dong-medicine Inula cappa[J]. Chin J Exp Tradit Med Form (中国实验方剂学杂志),2012,18(21):258-260.
WU P, LI M, GONG Z P, et al. Analysis of intestinal absorption characteristics of Inula cappa extract by in situ intestinal circulating perfusion model[J]. Chin J Exp Tradit Med Form (中国实验方剂学杂志),2018,24(2):1-8.
LOU Q. The study on tissue distribution and excretion of GDH in rats [D].Guangdong Pharmaceutical University(广东药学院),2015.
LIU L L, LI H, HE D, et al. Metabolic and Excretory Process of Citri Reticulatae Pericarpium Viride and Citri Reticulatae Pericarpium in Rats′ Urine and Feces Analyzed by RRLC-QqQ-MSn[J]. China J Chin Mater Med(中国中药杂志),2018,43(22):4519-4527.
GONG Z P, CHEN T T, HOU J Y, et al. Analysis of components in plasma from effective fractions of Inula cappa based on UHPLC/Q-TOF /MS[J]. Chin Pharmacol Bull(中国药理学通报),2017,33(11):1605-1610.
GONG Z P, HOU J Y, HOU J, et al. Metabolism of active fractions of Inula cappa in rat bile[J].Nat Prod Res Dev(天然产物研究与开发),2017,29(10):1700-1717.
GONG Z P, WU L L, WU P, et al. Analysis of metabolites of Inula cappa extract in rat feces[J]. Chin J Exp Tradit Med Form (中国实验方剂学杂志),2017,23(24):100-105.
WEN Z, XIA W, SI H F, et al. Chemical constituents of Inula cappa[J]. Chin Pharm J(中国药学杂志), 2017, 52(1):25-30.
TOMAS-BARBERAN F, GARCíA-VILLALBA R, QUARTIERI A, et al. In vitro transformation of chlorogenic acid by human gut microbiota[J]. Mol Nutr Food Res, 2014, 58(5):1122-1131.
LI Y, ZHOU M M, XIN L N, et al. Advances in study on gut flora metabolism of chlorogenic acid[J]. Chin Tradit Herb Drugs(中草药),2015,40(4):610-614.
DEL RIO D, STALMACH A, CALANI L, et al. Bioavailability of coffee chlorogenic acids and green tea flavan-3-ols[J]. Nutrients, 2010, 2(8):820-833
TANG L,FENG Q,ZHAO J, et al. Involvement of UDP-glucuronosyltranferases and sulfotransferases in the liverand intestinal first-pass metabolism of seven flavones in C57 mice and humans in vitro [J]. Food Chem Toxicol, 2012, 50(5):1460-1467.
LI J M, HE M Z,FENG Y L, et al. In-vivo studies on metabolism of luteolin and luteoloside in rats[J]. Tradit Chin Drug Res Clin Pharmacol(中药新药与临床药理),2017,28(1):61-68.