Abstract：OBJECTIVE To isolate four polysaccharides from Tremella and study their physicochemical properties, microstructures, antioxidant activities and anti-inflammation effects. METHODS The polysaccharides were purified using water extraction method, DEAE-Sepharose CL-6B and Sephadex G-100. The physicochemical properties and microstructures were studied using high-performance size-exclusion chromatography (HPSEC), gas chromatography (GC), Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM) analysis. Anti-inflammation activities of the polysaccharides were identified using real time quantitative PCR. RESULTS Four kinds of major polysaccharides (TABW-Ⅱ, TFBW-Ⅱ, TEPW-Ⅱ and TSPW-Ⅰ) were purified from water-extraction crude polysaccharides from Tremella. TABW-Ⅱ, TFBW-Ⅱ, TEPW-Ⅱ and TSPW-Ⅰ were homogeneous with relative molecular mass 26×103, 11×103, 21×103 and 356×103, respectively, and they consisted mainly of glucose and mannose. TEM reveale that their molecular morphologies were linear, and TSPW-Ⅰ had more extended structure with entangled chains. Compared with vitamin C (Vc), TSPW-Ⅰ showed more noticeable scavenging effects of superoxide (EC50=0.127 mg·mL-1) and hydroxyl (EC50=0.088 mg·mL-1) radicals (P<0.05). TSPW-Ⅰ also showed the highest inhibitory effects of TNF-α, IL-6 and COX-2 mRNA expressions in LPS-stimulated RAW 264.7 mouse macrophage cells, and the inhibitory effects were more than 95% at 150 μg·mL-1 TSPW-Ⅰ. CONCLUSION The results indicate that TSPW-Ⅰ with entangled and extended liner structures have more obvious antioxidant and anti-inflammation effects than other major polysaccharides from Tremella family. Furthermore, this study indicates the potential utilization of Tremella polysaccharides in anti-inflammatory agents.
王昭晶, 罗巅辉, 曾亚威. 4种银耳属多糖的理化特征、微观结构及其抗氧化和抗炎症作用研究[J]. 中国药学杂志, 2019, 54(21): 1788-1793.
WANG Zhao-jing, LUO Dian-hui, ZENG Ya-wei. Physicochemical Properties, Microstructure, and Antioxidant and Anti-inflammation Effects of Four Major Polysaccharides from Tremella. Chinese Pharmaceutical Journal, 2019, 54(21): 1788-1793.
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2019, Vol. 54 
