目的 制备共轭亚油酸-紫杉醇自组装纳米粒(conjugated linoleic acid-paclitaxel conjugate self-assembled nanoparticles, CLA-PTX NPs)。方法 本实验采用纳米沉淀法制备,通过动态光散射、核磁共振氢谱、拉曼光谱、傅里叶变换红外光谱及氮元素分布分析等方法对所制备的CLA-PTX NPs的表面和内部基团进行表征,确定CLA-PTX NPs的表面基团和内部基团,为阐述其自组装机制提供依据。结果 PTX中C-1位和C-7位的羟基、C-4位和C-10位的乙酰基位于CLA-PTX NPs的表面,CLA碳链、PTX中C-2位和C-3′位的苯环及C-3′位的酰胺键位于CLA-PTX NPs的内部。据此推测CLA-PTX的自组装方式是非极性的CLA碳链因疏水作用而自发向内聚集,PTX中亲水的含氧基团羟基、羰基等在纳米粒表面从而形成纳米粒。结论 CLA-PTX NPs表面基团的确定也为其可能的表面修饰提供参考和依据。更为重要的是,本实验还为其他自组装纳米粒结构的表征提供了相关可行的方法。
Abstract
OBJECTIVE To prepare, the conjugated linoleic acid-paclitaxel conjugate self-assembled nanoparticles (CLA-PTX NPs) by nanoprecipitation. METHODS The Dynamic light scattering, nuclear magnetic resonance spectroscopy, raman spectroscopy, fourier transform infrared spectroscopy and nitrogen element distribution of CLA-PTX NPs were studied. RESULTS The hydroxyl groups (C-4 and C-10 of PTX) and the acetyl groups (C-1 and C-7 of PTX) were on the surface of CLA-PTX NPs, CLA carbon chain, the benzene ring (C-2 and C-3′ of PTX) and the amide bond (C-3′ of PTX) were inside the CLA-PTX NPs. CONCLUSION It is speculated that the self-assembly of CLA-PTX is that the non-polar CLA carbon chain spontaneously aggregates inward due to hydrophobic interaction, and the hydrophilic oxygen-containing groups of PTX (hydroxyl group and carbonyl group) are on the surface of the nanoparticle to form nanoparticles.
关键词
共轭亚油酸-紫杉醇自组装纳米粒 /
结构 /
核磁共振氢谱 /
拉曼光谱 /
氮元素分布分析
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Key words
conjugated linoleic acid-paclitaxel conjugate self-assembled nanoparticle (CLA-PTX NPs) /
structure /
nuclear magnetic resonance spectroscopy /
Raman spectroscopy /
nitrogen element distribution analysis
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中图分类号：
R944
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脚注
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基金
国家自然科学基金项目资助(81773646);国家重点研发计划资助(2017YFA0205600);教育部创新团队项目资助(BMU2017TD003)
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