Abstract：OBJECTIVE To study the chemical constituents of the antioxidant active sites and establish the determination method of the total content of phenolic acid in Actinidia chinensis Planch. seeds. METHODS AB-8 macroporous adsorption resin, silica gel and ultra performance liquid chromatography-mass spectrometry (UPLC-MS/MS) were used to separate and purify the compounds, and the compounds were identified according to the properties and spectral data of the compounds. The total content of phenolic acid was determined by the spectrophotometry. To establish a method for determination of total content of phenolic acid using ferulic acid as a positive control and ferric chloride-potassium ferricyanide solution as a color developer and methodological investigation was carried out. RESULTS Twelve compounds were isolated and identified from the active parts of Actinidia chinensis Planch. seeds, which were protocatechuic acid (1), vanillic acid (2), syringic acid (3), protocatechuic aldehyde (4), 3,4-dihydroxyace to phenone. ethyl ketone (5), dihydroconiferyl alcohol (6), 4, 4-dihydroxydiphenylmethane (7), shikimic acid (8), ferulic acid (9), 3-acetoxymethyl-4-methoxy methyl benzoate (10), bis(4-methoxyphenyl) methane (11), and methyl caffeate (12). The total content of phenolic acid in kiwifruit seeds was determined by ultraviolet spectrophotometry. The linear range of the method was 1.6-16 μg·mL-1.The regression equation is Y=0.087 8x+0.045 8, r2=0.999 3, the average recovery is 99.36%, and the RSD is 2.869 9%.The total content of phenolic acid in the total extract was 6.61%, and the active site was 42.46%. The total content of phenolic acid in the active site is about 7 times that of the total extract. It can be seen from the results that the extraction process can effectively enrich the phenolic acid compounds. CONCLUSION Compounds 5-7 and 10-12 are isolated from the plant for the first time. The determination method of total content of phenolic acid is simple,fast,stable and reproducible,which provides a scientific basis for the systematic study of phenolic acid compounds in kiwifruit seeds.
张珊, 宋乐园, 陈林玉, 王云雨, 商冲, 毕跃峰. 中华猕猴桃籽中抗氧化化学成分研究及总酚酸含量测定[J]. 中国药学杂志, 2019, 54(20): 1653-1659.
ZHANG Shan, SONG Le-yuan, CHEN Lin-yu, WANG Yun-yu, SHANG Chong, BI Yue-feng. Antioxidant Chemical Composition and Determination of Total Content of Phenolic Acid in Seeds of Actinidia chinensis Planch. Chinese Pharmaceutical Journal, 2019, 54(20): 1653-1659.
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2019, Vol. 54 
