Research Progress on the Effective Substances and the Mechanism of Traditional Chinese Medicine Compound
LI Fei1,4, ZHAO Yuan2, LIN Rui1, ZHAO Mei-na1, SU Jing1, WEN Ai-dong1*, XI Miao-miao1,3*
1. Department of Pharmacy, Xijing Hospital, Medical University of The Air Force, Xi′an 710032, China; 2. Department of Pharmacology, Chengde Vocational Nursing College, Chengde 067000, China; 3. Xi'an Tiangong Biomedical Research Institute, Xi′an 710032, China; 4. Department of Pharmacy, The Hospital of 92012 Troops, PLA Navy, Zhoushan 316000, China
Abstract:It is important to elucidate the effective substances and the mechanism of Traditional Chinese Medicine (TCM) compound in revealing the compatibility mechanism of TCM, completing the transformation of large varieties of TCM, modernizing TCM, and gaining international recognition. The research METHODS mainly include: the serum pharmacochemistry, network pharmacology, spectrum-effect relationship, high throughput screening, metabonomics and so on. The main research METHODS reviewed through domestic and foreign related literatures can provide the reference for elucidating the effective substances and the mechanism of TCM compound.
李飞, 赵原, 蔺瑞, 赵美娜, 苏晶, 文爱东, 奚苗苗. 中药复方药效物质及作用机制研究进展[J]. 中国药学杂志, 2019, 54(13): 1037-1044.
LI Fei, ZHAO Yuan, LIN Rui, ZHAO Mei-na, SU Jing, WEN Ai-dong, XI Miao-miao. Research Progress on the Effective Substances and the Mechanism of Traditional Chinese Medicine Compound. Chinese Pharmaceutical Journal, 2019, 54(13): 1037-1044.
MU F, DUAN J L, WEN A D, et al. Application of metabonomics in the study of efficacy material base and mechanism of traditional Chinese medicine in the treatment of coronary heart disease[J]. Chin J New Drugs(中国新药杂志), 2015, 24(19):2197-2202.
[2]
SONG H P, YANG H, GAO W, et al. Advances in key techniques for the discovery of active ingredients in traditional Chinese medicine[J]. World Sci Technol Mod Tradit Chin Med Mater Med(世界科学技术-中医药现代化), 2016, 18(7):1093-1098.
[3]
LUO G A, XIE Y Y, WANG Y M, et al. Research on the modernization of precision medicine and traditional Chinese medicine--five on the establishment of new medicine[J]. World Sci Technol Mod Tradit Chin Med Mater Med(世界科学技术-中医药现代化), 2017, 19(1):19-29.
[4]
LUO G A, WANG Y M. The research method and theory of the effective part of TCM compound prescription[J]. Chin Tradit Pat Med(中成药), 1997, 19(8):44-45.
[5]
HOOD L, HEATH J R, PHELPS M E, et al. Systems biology and new technologies enable predictive and preventative medicine[J]. Science, 2004, 306(5696):640-643.
[6]
LUO G A, LIANG Q L, ZHANG R L, et al. Chemical composition and traditional Chinese medicine--it also analyzes the material basis of kailing compound[J]. World Sci Technol Mod Tradit Chin Med Mater Med(世界科学技术-中医药现代化), 2006, 8(1):6-15.
[7]
LUO G A, LIANG Q L, LIU Q F, et al. Integrated holistic systems biology of chemical composition--a holistic methodology for the study of compatibility and mechanism of action of TCM compounds[J]. World Sci Technol Mod Tradit Chin Med Mater Med(世界科学技术-中医药现代化), 2007, 9(1):10-15.
[8]
FU J H, FU Y, LIU J X. Conception of the "component Chinese medicine" hypothesis[J]. Chin J Inf Tradit Chin Med(中国中医药信息杂志), 2006, 13(1):52-54.
[9]
LI A X. The proposal of "pharmacodynamic property hypothesis" of traditional Chinese medicine[J]. Tianjin Pharm(天津药学), 2007, 19(2):41-44.
[10]
WANG X J. The formation and development of Chinese medicine serum pharmacochemistry[J]. World Sci Technol Mod Tradit Chin Med Mater Med(世界科学技术-中医药现代化), 2010, 12(4):632-633.
[11]
XU F, YANG D H, SHANG M Y, et al. "Obvious form", "superposition effect" and "toxic dispersion effect" of medicinal materials--reflections on metabolism of traditional Chinese medicine[J]. World Sci Technol Mod Tradit Chin Med Mater Med(世界科学技术-中医药现代化), 2014, 26(4):688-703.
[12]
ZE L M G, ZHANG X F, SU D N, et al. Application status of serum pharmacochemistry in traditional Chinese medicine[J]. J China Pharm(中国药房), 2017, 28(7):978-981.
[13]
WANG X J. Chinese Medicine Serum Pharmacochemistry(中药血清药物化学)[M]. Beijing: Science Press, 2010: 42.
[14]
MA F X, XUE P F, WANG Y Y, et al. Research progress of serum pharmacochemistry of traditional Chinese medicine[J]. China J Chin Mater Med(中国中药杂志), 2017, 42(7):1265-1270.
[15]
AN R, LI Y, LI M, et al. Plasma pharmacochemistry combined with pharmacokinetics and pattern recognition analysis to screen potentially bioactive components from Daming capsule using ultra high performance liquid chromatography with electrospray ionization quadrupole time-of-flight mass spectrometry[J]. J Sep Sci, 2015, 38(9):1507-1514.
[16]
LI X, ZHANG A, SUN H, et al. Rapid discovery of absorbed constituents and metabolites in rat plasma after the oral administration of Zi Shen Wan using high-throughput UHPLC-MS with a multivariate analysis approach[J]. J Sep Sci, 2016, 39(24):4700-4711.
[17]
HAN Y, WU F, ZHANG A, et al. Characterization of multiple constituents in rat plasma after oral administration of Shengmai San using ultra-performance liquid chromatography coupled with electrospray ionization/quadrupole-time-of-flight high-definition mass spectrometry[J]. Anal Methods, 2015, 7(3):830-837.
[18]
LIU C, ZHANG A, HAN Y, et al. Ultra-high performance liquid chromatography coupled with time-of-flight mass spectrometry screening and analysis of potential bioactive compounds from traditional chinese medicine Kai-Xin-San, using a multivariate data processing approach and the MetaboLynx tool[J]. Rsc Adv, 2015, 5(1):85-92.
[19]
FAN Y, MAN S, LI H, et al. Analysis of bioactive components and pharmacokinetic study of herb-herb interactions in the traditional Chinese patent medicine Tongmai Yangxin Pill[J]. J Pharm Biomed, 2016, 120: 364-373.
[20]
LIU Y, WEI W F, HUO J H, et al. Serum pharmacochemistry of Qinbai Qingfei concentrated pellets based on UPLC-Q-TOF-MS[J]. China J Chin Mater Med(中国中药杂志), 2017, 42(3):572-579.
[21]
XIANG W Y, YANG W, MEI C Y, et al. Serum pharmacochemistry study on Huafengdan[J]. J China Pharm(中国药房), 2016, 27(28):3911-3914.
[22]
MA H, LIU Y, MAI X, et al. Identification of the constituents and metabolites in rat plasma after oral administration of Huanglian Shangqing pills by ultra high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry[J]. J Pharm Biomed Anal, 2016, 125: 194-204.
[23]
ZHU H, BI K, HAN F, et al. Identification of the absorbed components and metabolites of Zhi-Zi-Da-Huang decoction in rat plasma by ultra-high performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry[J]. J Pharm Biomed, 2015, 111: 277-287.
[24]
XU W, WU X, HUANG M Q, et al. Analysis on pharmacodynamic constituents in Gualou Guizhi Decoction by serum pharmacochemistry[J]. Chin Tradit Herb Drugs(中草药), 2017, 48(10):2033-2043.
[25]
SUN H, SUN W, ZHANG A, et al. Chapter 5:Analytical Characterization of Yin-Chen-Hao-Tang by Serum Pharmacochemistry of TCM, Serum Pharmacochemistry of Traditional Chinese Medicine[M]. London:Mica Haley, 2017.
[26]
PAN T, OU Y B, XIAO Z Q, et al. Identification and assay of constituents of Chankangle Granules migrating to blood by HPLC-DAD[J]. Chin Hosp Pharm J(中国医院药学杂志), 2016, 36(11):908-911.
[27]
ZHU T M, DUAN X Y, JIANG N, et al. Serum pharmacochemistry of compound Kudai decoction[J]. Cent South Pharm(中南药学), 2016, 14(9):944-947.
[28]
WU J M, ZHANG Z R, ZHANG H C, et al. Study on serum pharmacochemistry of Keluoxin capsule[J]. World Sci Technol Mod Tradit Chin Med Mater Med(世界科学技术-中医药现代化), 2014, 16(8):1784-1788.
[29]
MU J, GAO X, LI Q, et al. Vortex-ultrasound-assisted dispersive liquid-liquid microextraction coupled with gas chromatography-mass spectrometry for the analysis of volatile bioactive components and comparative pharmacokinetic study of the herb-herb interactions in Guanxin Shutong Capsule[J]. J Sep Sci, 2017, 40(16):3267-3278.
[30]
CHEN Z, PAN M J, XING X F, et al. Research progress on serum pharmacochemistry studies in pharmacodynamics material basis of Chinese materia medica and Chinese herbal formula[J]. Drug Eval Res, 2016, 39(1):143-147.
[31]
HOPKINS A L. Network pharmacology: the next paradigm in drug discovery[J]. Nat Chem Biol, 2008, 4(11):682-690.
[32]
SONG K K, BI T, ZHAN X R, et al. Strategies for the discovery of active ingredients in traditional Chinese medicine under the guidance of network pharmacology[J]. World Sci Technol Mod Tradit Chin Med Mater Med(世界科学技术-中医药现代化), 2014, 16(1):27-31.
[33]
PANG H Q, YUE S J, TANG Y P, et al. Integrated metabolomics and network pharmacology approach to explain possible action mechanisms of Xin-Sheng-Hua Granule for treating anemia[J]. Front Pharmacol, 2018, 9: 165.
[34]
CHEN L, CAO Y, ZHANG H, et al. Network pharmacology-based strategy for predicting active ingredients and potential targets of Yangxinshi tablet for treating heart failure[J]. J Ethnopharmacol, 2018, 219: 359-368.
[35]
GAO L, WANG K X, ZHOU Y Z, et al. Uncovering the anticancer mechanism of Compound Kushen Injection against HCC by integrating quantitative analysis, network analysis and experimental validation[J]. Sci Rep, 2018, 8(1):624.
[36]
WANG Y, ZHAO Y, JIANG W, et al. iTRAQ-Based proteomic analysis reveals recovery of impaired mitochondrial function in ischemic myocardium by Shenmai Formula[J]. J Proteome Res, 2018, 17(2):794-803.
[37]
LIU X, SHEN J, FAN D, et al. Yupingfeng San inhibits NLRP3 inflammasome to attenuate the inflammatory response in asthma mice[J]. Front Pharmacol, 2017, 8: 944.
[38]
ZENG L, YANG K, GE J. Uncovering the pharmacological mechanism of astragalus salvia compound on pregnancy-induced hypertension syndrome by a network pharmacology approach[J]. Sci Rep, 2017, 7(1):16849.
[39]
WU J R, JIN Y P, WANG K H, et al. Mechanism of lonicerae japonicae flos-weeping forsythia herbal pair based on network pharmacology[J]. Chin J Exp Tradit Med Form(中国实验方剂学杂志), 2017, 23(5):179-183.
[40]
DENG X, XING X, SUN G, et al. Guanxin Danshen Formulation protects against myocardial ischemia reperfusion injury-induced left ventricular remodeling by upregulating estrogen receptor beta[J]. Front Pharmacol, 2017, 8: 777.
[41]
HONG M, LI S, TAN H Y, et al. A network-based pharmacology study of the herb-induced liver injury potential of traditional hepatoprotective Chinese herbal medicines[J]. Molecules, 2017, 22(4):632.
[42]
FANG H Y, ZENG H W, LIN L M, et al. A network-based method for mechanistic investigation of Shexiang Baoxin Pill′s treatment of cardiovascular diseases[J]. Sci Rep, 2017, 7: 43632.
[43]
ZENG L, YANG K. Exploring the pharmacological mechanism of Yanghe Decoction on HER2-positive breast cancer by a network pharmacology approach[J]. J Ethnopharmacol, 2017, 199(5):68-85.
[44]
WANG N, ZHENG Y, GU J, et al. Network-pharmacology-based validation of TAMS/CXCL-1 as key mediator of XIAOPI formula preventing breast cancer development and metastasis[J]. Sci Rep, 2017, 7(1):14513.
[45]
BROACH J R, THORNER J. High-throughput screening for drug discovery[J]. Nature, 1996, 384(6604 suppl):14-16.
[46]
ZHANG D S. The application of them uniform design-high throughput on screening antioxidant research of 17 components Radix Salviae Miltiorrhizae and compound Danshen Tablets[J]. Acta Neuropharmacologica(神经药理学报), 2012, 2(6):1-17.
[47]
LI R, YAN Z Y, LI W J, et al. To establish the relationship between Chinese traditional medicine (TCM)[J]. Educ Tradit Chin Med(中医教育), 2002, 21(2):62.
[48]
CAI L, ZHANG Q, YANG F Q. Applications progress on spectrum-effect relationship in study of Chinese materia medica[J]. Chin Tradit Herb Drugs(中草药), 2017, 48(23):5005-5011.
[49]
XIE R F, ZHOU X, SHI Z N, et al. Study on spectrum-effect relationship of rhizoma Rhei, cortex Magnoliae Officinalis, fructus Aurantii Immaturus and their formula[J]. J Chromatogr Sci, 2013, 51(6):524-532.
[50]
WANG Y, LI Y, ZHANG Y, et al. Multi-dimensional spectrum-effect relationship of the impact of Chinese herbal formula Lichong Shengsui Yin on ovarian cancer[J]. Molecules, 2017, 22(6):979.
[51]
DOU Z H, LUO L, HOU J Y, et al. Hepatoprotective ingredients of Yinchenhao Decoction based on the time-spectrum-effect relationship of serum containing drugs[J]. Chin J Hosp Pharm(中国医院药学杂志), 2017, 37(13):1232-1237.
[52]
CHENG L, ZHENG J, XU P H, et al. Spectrum-effect relationship of anti-hepatic fibrosis effect of Liuwei Wuling Tablets[J]. Chin Tradit Pat Med(中成药), 2016, 38(4):820-825.
[53]
ZHAO X L, LING J J, WEI J B. HPLC Fingerprint analysis of the extract from different batches of Sini decoction and relation of fingerprint with pharmacodynamics in anti myocardial ischemic effects[J]. Northern Pharm(北方药学), 2015, 12(12):98-100.
[54]
YAN H Y, ZOU C C, WEI M L, et al. Spectrum-effect relationship of Gualou Xiebai Dropping Pills on myocardial ischemia[J]. J Chin Med Mater(中药材), 2015, 38(9):1912-1918.
[55]
LIU T, WANG H D, DI L Q, et al. HPLC Specific chromatogram spectrum-effect relationship for Shuanghuanglian on MDCK cell injury induced by influenza A virus (H1N1)[J]. China J Chin Mater Med(中国中药杂志), 2015, 40(21):4194-4199.
[56]
ZHANG F, ZHAN Q, DONG X, et al. Shengxian decoction in chronic heart failure treatment and synergistic property of Platycodonis Radix: a metabolomic approach and its application[J]. Mol Biosyst, 2014, 10(8):2055-2063.
[57]
ZHOU Y Z, WANG Y, RONG J F, et al. Application of metabolomics in TCM syndrome differentiation of coronary heart disease[J]. Chin J Integr Med Cardio-/Cerebrovasc Dis(中西医结合心脑血管病杂志), 2016, 14(15):1738-1741.
[58]
MA F X, XUE P F, WANG Y Y, et al. Research progress of serum pharmacochemistry of traditional Chinese medicine [J]. China J Chin Mater Med(中国中药杂志), 2017, 42(7):1265-1270.
[59]
XIN X, ZOU H, ZHENG N, et al. Metabonomic strategy to the evaluation of chinese medicine compound danshen dripping pills interfering myocardial ischemia in rats[J]. Evid Based Complement Alternat Med, 2013, 2013(3):718305.
[60]
LIU P, WANG P. Application of metabolomics technology in the research of Chinese medicine[J]. Chin J Integr Med(中国中西医结合杂志 英文版), 2014, 20(4):307-310.
[61]
WANG N, ZHAO X, HUAI J, et al. Arachidonic acid metabonomics study for understanding therapeutic mechanism of Huo Luo Xiao Ling Dan on rat model of rheumatoid arthritis[J]. J Ethnopharmacol, 2018, 217: 205-211.
[62]
WEI Z, XU C, LIU S, et al. Metabonomics study of the effects of traditional Chinese medicine formula Ermiaowan on hyperuricemic rats[J]. J Sep Sci, 2018, 41(2):560-570.
[63]
MU F, DUAN J, BIAN H, et al. Metabonomic strategy for the evaluation of Chinese medicine salvia miltiorrhiza and dalbergia odorifera interfering with myocardial ischemia/reperfusion injury in rats[J]. Rejuven Res, 2017, 20(4):263-277.
[64]
SHUI S, CAI X, HUANG R, et al. Metabonomic analysis of serum reveals antifatigue effects of Yi Guan Jian on fatigue mice using gas chromatography coupled to mass spectrometry[J]. Biomed Chromatogr, 2018, 32(2):e4085.
[65]
GUO Y, LI Z, LIU X, et al. 1H-NMR-Based metabonomic study of functional dyspepsia in stressed rats treated with Chinese medicine Weikangning[J]. Evid Based Compl Alter Med, 2017, 2017(3):1-18.
[66]
JIANG M, WANG Q, CHEN J, et al. Comparative metabonomics of Wenxin Keli and Verapamil reveals differential roles of gluconeogenesis and fatty acid beta-oxidation in myocardial injury protection[J]. Sci Rep, 2017, 7(1):8739.
[67]
LI P, LIAO S T, WANG J S, et al. Protection by Huang-Lian-Jie-Du decoction and its constituent herbs of lipopolysaccharide-induced acute kidney injury[J]. Febs Open Bio, 2017, 7(2):221-236.
[68]
SHUI S, CAI X, HUANG R, et al. The investigation of anti-inflammatory activity of Yi Guanjian decoction by serum metabonomics approach[J]. J Pharm Biomed Anal, 2017, 133(10):41-48.
[69]
TANG F, LIANG S Y, TIAN Y X, et al. Predicting effective components and targets of essential oil of Asari Radix et Rhizoma on allergic rhinitis[J]. Chin J Exp Tradit Med Form (中国实验方剂学杂志), 2015, 21(24):126-131.
[70]
ZHOU X, ZHANG A, WANG L, et al. Novel chinmedomics strategy for discovering effective constituents from ShenQiWan acting on ShenYangXu syndrome[J]. Chin J Nat Med, 2016, 14(8):561-581.
[71]
ZHOU W B, LIN L, LI Z Y, et al. Study on active ingredient and mechanism in preventing vascular dementia of Tianzhusan coming from Tujia medicine[J]. China J Chin Mater Med(中国中药杂志), 2015, 40(13):2668-2673.
[72]
XIANG Z, SUN H, CAI X, et al. The study on the material basis and the mechanism for anti-renal interstitial fibrosis efficacy of rhubarb through integration of metabonomics and network pharmacology[J]. Mol Biosyst, 2015, 11(4):1067-1078.
[73]
GAO K, YANG R, ZHANG J, et al. Effects of Qijian mixture on type 2 diabetes assessed by metabonomics, gut microbiota and network pharmacology[J]. Pharmacol Res, 2018, 130: 93-109.
[74]
ZHANG Z, LU F, LIU H, et al. An integrated strategy by using target tissue metabolomics biomarkers as pharmacodynamic surrogate indices to screen antipyretic components of Qingkaikling injection[J]. Sci Rep, 2017, 7(1):6310.
[75]
XIANG Z, SUN H, CAI X, et al. The study on the material basis and the mechanism for anti-renal interstitial fibrosis efficacy of rhubarb through integration of metabonomics and network pharmacology[J]. Mol Biosyst, 2015, 11(4):1067-1078.
[76]
GUO Q, TIAN C W, REN T, et al. Progress in basic research on the pharmacodynamic substances of traditional Chinese medicine[J]. World Sci Technol Mod Tradit Chin Med Mater Med(世界科学技术-中医药现代化), 2015, 17(3):648-654.