Identification of Uygur Herb Capparis spinose Based on Sequences of the Plastid psbA-trnH Intergenic Regions
CHENG Bo1, YANG Wei-jun1, LIU Huan-huan1,2, HE Jiang1*
1. Key Laboratory of Xinjiang Uighur Medicine, Xinjiang Institute of Materia Medica, Urumqi 830004, China;
2. College of Pharmacy, Xinjiang Shihezi University, Shihezi 832003, China
OBJECTIVE To provide a reference for molecular biology identification of C. spinosa by comparing and analyzing the sequence of psbA-trnH gene intergenic regions between Capparis spinosa L. and others 8 medicinal plants. METHODS Total DNA were extracted, psbA-trnH intergenic regions sequences were isolated using PCR amplification, and sequence analysis, evaluation of intraspecific and interspecific genetic distance used the Kimura 2 parameter(K2P)model as well as construction of phylogenetic tree based on UPGMA were conducted by the MEGA7. RESULTS Compared with C. spinosa, the interspecific genetic distance is 0.045-0.474. The average is 0.238. The interspecific minimum was larger than the intraspecific maximum. The results of UPGMA tree indicated every species was sorted out and C. spinosa was distinguished from the others effectively. CONCLUSION The sequences of chloroplast psbA-trnH gene intergenic regions is valuable for the identification and study of C. spinosa.
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CHENG Bo, YANG Wei-jun, LIU Huan-huan, HE Jiang. Identification of Uygur Herb Capparis spinose Based on Sequences of the Plastid psbA-trnH Intergenic Regions. Chinese Pharmaceutical Journal, 2019, 54(12): 965-970.
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2019, Vol. 54 
