Optimization of Extraction Process for Active Ingredients in Seeds of Sophora alopecuroides by Plackett-Burman Design and Response Surface Analysis
MA Bing-zhi1, LI Li2, HE Jun1, LI Dong1, ZHANG Shu-jun1, LIANG Ying-ying1, WANG Hai-yang1, MA Shu-wei3, LIU Xiao1*
1. Department of Pharmacy, China-Japan Friendship Hospital, Beijing 100029, China;
2. Beijing Key Laboratory of Plant Resource Research and Development, Beijing 100048, China;
3. School of Chinese Materia Medica, Beijing University of Traditional Chinese Medicine, Beijing 102488, China
OBJECTIVE To optimize extraction process for active ingredients in seeds of Sophora alopecuroides, to provide a reference for scale production. METHODS Active ingredients from Sophora alopecuroides were extracted by ethanol, with average yield of oxysophocarpine and oxymatrine as index, some factors affecting index were firstly evaluated by Plackett-Burman design, then taking oxysophocarpine and oxymatrine as indexes respectively, extraction conditions were optimized by Box-Behnken design, experimental data was fitted by multiple linear regression and binomial formula fitting, extraction process was optimized by response surface method, and prediction was carried out through comparing the observed and predicted value. RESULTS Extracting times, crushing degree and solvent times had significant effects on yields of oxysophocarpine and oxymatrine; binomial equation fitted well with good predictability. optimum extraction technology of Sophora alopecuroides was as following:crushed through 65 mesh sieve, extracted 4 times with 12-fold the amount of 60% ethanol for 2 h each time; yield of oxysophocarpine and oxymatrine was 92.3%, 78.6% respectively, both deviations were small by comparing with the predicted value. CONCLUSION This extraction process is reasonable and feasible by Plackett-Burman design and response surface analysis with good predictability. This study can provide experimental basis for further scale production of Sophora alopecuroides.
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MA Bing-zhi, LI Li, HE Jun, LI Dong, ZHANG Shu-jun, LIANG Ying-ying, WANG Hai-yang, MA Shu-wei, LIU Xiao. Optimization of Extraction Process for Active Ingredients in Seeds of Sophora alopecuroides by Plackett-Burman Design and Response Surface Analysis. Chinese Pharmaceutical Journal, 2019, 54(12): 953-959.
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2019, Vol. 54 
