1. Pharmacy College, Chengdu University of Traditional Chinese Medicine, Key Laboratory Breeding Base of Systematic Research and Utilization on Chinese Material Medical Resources Co-founded by Sichuan Province and Ministry of Science and Technology, Chengdu 611137, China; 2. Central Laboratory, Teaching Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610072, China; 3. Chengdu Food and Drug Control, Chengdu 610045, China; 4. Sanajon Pharmaceutical Group, Chengdu 610000, China
Abstract��OBJECTIVE To reveal the phenomenon of component transformation during the heating reflux extraction of Phyllanthus emblica L. as described in the Chinese Pharmacopoeia, study its transformation mechanism, and propose a new Phyllanthus emblica extraction method.METHODS HPLC fingerprints were used to track the changes of various tannins in Phyllanthus emblica L. during the process of heating reflux extraction, and model solution was used to reveal the transformation mechanism. The pharmacopoeia method was used to track the changes of gallic acid content in the heating reflux extraction process of Phyllanthus emblica L.. Finally, ultrasonic extraction was used as a new extraction method and the process was optimized. RESULTS The fingerprints showed that the contents of chebulagic acid, corilagin and gallocatechin decreased significantly during the heating reflux extraction process, while gallic acid, chebulic acid and ellagic acid increased significantly. The results of model solution showed that during the hydrolysis process, corilagin hydrolyzed to produce gallic acid and ellagic acid. While chebulagic acid hydrolyzed to produce one molecule of corilagin and one molecule of chebulic acid. And then the corilagin continued to hydrolyze. The tracking survey using pharmacopoeia reflux extraction method showed that with the increase of reflux time, the content of gallic acid was increasing. The best optimization result of ultrasonic extraction was as followsextraction time 20 min, extraction power 300 W, solvent volume 30 mL, extraction 1 time. CONCLUTION The hydrolysis of hydrolyzable tannins contained in the Phyllanthus emblica L. during the heating reflux extraction process, such as chebulagic acid and corilagin, may cause the increase of detected gallic acid content, which may affect the accuracy of the detection results. Ultrasonic extraction can inhibit the hydrolysis of tannins, which can ensure the accuracy of the determination results of gallic acid.
�ƺ���, κϦ��, �ֿ�֥, ̷��, ������, ����, �Ŷ���. ����ӻ�������������ת����ҩ�京���ⶨ����������̽��[J]. �й�ҩѧ��־, 2019, 54(7): 581-587.
HUANGHao-zhou, WEIXi-chuan, LINJun-zhi, TANPeng, FANSan-hu, HANLi, ZHANGDing-kun. Tannin Transformation During the Reflux Process of Phyllanthus emblica L. and Discussion of the Content Determination Method in Chinese Pharmacopoeia. Chinese Pharmaceutical Journal, 2019, 54(7): 581-587.
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