Abstract��OBJECTIVE To compare the chemical compositions of the stems (STL) and leaves (LTL) of Tussilago farfara L. using NMR-based metabolomic approach. METHODS The STL and LTL were analyzed by NMR, then the differential components were determined by multivariate statistical methods, including PCA, PLS-DA, OPLS-DA, and univariate analysis. RESULTS Fifty compounds were tentatively identified in the NMR spectra of STL and LTL. Multivariate coupled with univariate analysis revealed that some metabolites, such as valine,leucine,isoleucine,proline,chlorogenic acid,3,5-O-dicaffeoylquinic acid, 3,4-O-dicaffeoylquinic acid, and tussilagone,were present at higher levels in the LTF, while the STF contained higher levels of ��-glucose and ��-glucose.For some metabolites, such as malic acid, sucrose and choline, no significant differences were observed between STF and LTF. CONCLUSION This study reveals the chemical differences between STF and LTF in a holistic way, and lays the scientific foundation for the resource utilization of the stems and leaves of T. farfara L.
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QIYan-shuang, LIJing, GUODong, WANGYu-long, LEIZhen-hong, QINXue-mei, LIZhen-yu. Chemical Comparison of the Stems and Leaves of Tussilago farfara L. Using NMR-based Metabolomics. Chinese Pharmaceutical Journal, 2019, 54(8): 608-613.
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2019, Vol. 54 
