Abstract��Calculus Bovis is one precious traditional Chinese medicine used widely in clinical practice. Because of its high price and lack of resources, the substitutes of Calculus Bovis artificial Calculus Bovis, in vivo cultured Calculus Bovis and in vitro cultured Calculus Bovis also have been widely used in clinical practice. In order to understand the difference in chemical composition between Calculus Bovis and its substitutes, and determine whether all kinds of Calculus Bovis medicinal materials have special detection methods, the chemical constituents and quality control methods of Calculus Bovis and its substitutes were reviewed in this paper. Through the review of literature it was found that artificial Calculus Bovis mainly contain hyodeoxycholic acid and only a small amount of free bilirubin, and the chemical components of the other three kinds of Calculus Bovis medicinal materials are different. The chemical compositions of natural Calculus Bovis and in vitro cultured Calculus Bovis are similar, but the proportions of different types of bilirubin are different. At present, no characteristic ingredients of natural Calculus Bovis have been found to distinguish it from in vitro cultured Calculus Bovis and in vivo cultured Calculus Bovis.
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LIUWen-xi, CHENGXian-long, GUOXiao-han, HUXiao-ru, LIUYue, WEIFeng, MAShuang-cheng. Progress in Research on Chemical Constituents and Quality Control Methods of Calculus Bovis and Its Substitutes. Chinese Pharmaceutical Journal, 2019, 54(8): 597-602.
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2019, Vol. 54 
