Abstract��OBJECTIVE To study the preparation technology of baicalin glycosides liposomes. METHODS Baicalin liposomes modified by vitamin E and Tween 80 were prepared by reverse phase evaporation method. The free drug was separated by ultracentrifugation and the encapsulation rate was determined by UV spectrophotometry. Based on the results from single factor tests, orthogonal experimental design was used to investigate the factors influencing the envelopment rate of liposomes. The baicalin liposomes prepared by the optimized process were characterized for particle size, Zeta potential, and morphology. RESULTS The optimum conditions for the preparation of liposomes were as follows: the ratio of water phase to organic phase 1��3, the concentration of baicalin 3 mg��mL-1, the ratio of cholesterol to soy lecithin 1��6, dosage of vitamin E 2 mg,dosage of Tween 80 120 ��L. The mean diameter was 52.2 nm, Zeta potential was -51.9 mV, the encapsulation rate was 70.22% and drug loading capacity was 3.18%. Transmission Electr Microscope(TEM) results showed that the shape of the liposomes was good, the particle size was relatively uniform and consistent with the results of laser granulometry. CONCLUSION The stability of baicalin liposomes can be improved by the addition of vitamin E and Tween 80.
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2019, Vol. 54 
