HPLC Analysis of Six Iridoid Glycosides from Cymbaria dahurica L.
WU-REN-CAO-BU-DAO1, WANG Qing-hu2*, NA-REN-CHAO-KE-TU2, BI-LI-GE-TU2, BAO Wen-qiang2
1. Affiliated Hospital, Inner Mongolia University for Nationalities, Tongliao 028000, China; 2. College of Traditional Mongolian Medicine, Inner Mongolia University for Nationalities, Tongliao 028000, China
Abstract��OBJECTIVE To develop a method for simultaneous determination of six iridoid glycosides from Cymbaria dahurica.METHODSCymbaria dahurica collected from different regions were determined by HPLC. The separation was performed on Inertsil ODS-SP column (4.6 mm��250 mm,5 ��m) at 30 �� by gradient elution with CH3OH-H2O as the mobile phase, with the detection wavelength set at 210 nm.RESULTS Under the chromatographic conditions adopted in this study, all the calibration curves exhibited good linearity (r>0.999 4) in a relatively wide concentration range. The recovery of the method was within the range of 95.0%-98.0%, and the RSD was less than 3%. The contents of all the compounds (S1-S6) in samples from different habitats varied significantly.CONCLUSION The quantitative method for evaluating the quality of Cymbaria dahurica is established and can be used for the quality control of Cymbaria dahurica.
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2018, Vol. 53 
