Preparation of Site-specific Smart Nanocomposites for Drug Delivery and Study on Light-Controlled Cytarabine Release Performance
WANG Zhi-ping1, CHI Ze-feng2, CHEN Tie-yu3*
1. Shanxi Health Vocational College, Taiyuan 030619, China; 2. Pharmacy Department of No.8 People��s Hospital, Taiyuan 030012, China; 3. Pharmaceutical College Guangxi Medical University, Nanning 530021, China
Abstract��OBJECTIVE To prepare smart & site-specific drug carrier for controlled release purpose and study the bio-compatibilities and release performance.METHODS By using high pressure thermo-heat method in autoclave, superparamagnetic core was obtained and further coated by SiO2 and MCM-41, therefore the ��core-shell�� structure was formed. To make the carrier ��smart�� and thus responsive to stimuli which was light in this research, the tunnels of the molecular sieve were grafted with gating molecules, 4,5-diazafluoren-9-one (indicated in the paper as DAFO). For bio-compatibilities testing, MTT in-vitro experiment was conducted. Cytarabine was used as test drug to preliminarily evaluate the controlled release performance of the drug carrier in vitro.RESULTS The Fe3O4 nano-particles synthesized via high-pressure hydro-thermo procedure exhibited superparamagnetic with mean diameter of 280 nm. After SiO2 & molecular sieve coating steps and ligand grafting steps, the particles grew to 540 nm. The sub-structure of the carrier was confirmed by scanning/transmission electron microscope(SEM & TEM) and nitrogen adsorption/desorption. Our ��smart�� carrier was able to be guided to the sites or organs with magnetic field and more importantly it was able to unload drug molecules under 510 nm light irritation that could flip the gating-molecule. Furthermore, the drug carrier illustrated bio-compatibility and showed obvious cytotoxicity.CONCLUSION The novel nanocomposites developed in this study can be used as targeted drug carrier.
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WANG Zhi-ping, CHI Ze-feng, CHEN Tie-yu. Preparation of Site-specific Smart Nanocomposites for Drug Delivery and Study on Light-Controlled Cytarabine Release Performance. Chinese Pharmaceutical Journal, 2018, 53(24): 2102-2107.
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2018, Vol. 53 
