1. School of Pharmacy, Zhejiang University of Technology, Hangzhou 310014, China; 2. Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine,Nanjing 210028, China
Abstract��OBJECTIVE To investigate the effect of geniposide phospholipid complex on the degradation of geniposide by intestinal microflora, which provides the rationale for the design of an appropriate geniposide dosage form.METHODS The intestinal flora was collected from fresh rat feces. The geniposide and geniposide physical mixtures and their complexes were incubated with the rat intestinal flora in anaerobic conditions, respectively. Samples were taken at 0,2, 4, 6, 8 and 12 h. The content of geniposide was determined by HPLC. The preparative geniposide phospholipid complexes were characterized by DSC and X-ray diffraction.RESULTS In the physical mixture of geniposide and geniposide phospholipids, geniposide continuously decreased in the first 2 h, while the degradation accelerated at 2 to 4 h and completely degraded at 4 h. In the phospholipid complex group, the content of geniposide was decreased by 67.2% within 6 h, and it was completely degraded at 8 h.CONCLUSION The geniposide can be completely degraded in the gut microbiota of male SD rats in vitro. After the preparation of the phospholipid complex, the degradation rate of geniposide was slowed down. In the physical mixture group, the presence of phospholipids does not delay the degradation of jasminoidin.
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2018, Vol. 53 
