Abstract��OBJECTIVE To investigate the antioxidant ability and immune adjustment function of Sanguis Draconis flavones (SDF) on D-galactose aging in rats.METHODS The healthy SD rats were randomly divided into 6 groups: blank control group, aging model group, positive control group, low-dose, medium-dose and high-dose of SDF groups. Aging model was induced by subcutaneously injecting rats with 5% D-galactose. Rats of each group received daily administration by gavage for 6 weeks and the rats were weighted once per week in experimental period. The thymus and spleen index were measured; meanwhile, the contents of MDA, PC, AGES and the activity of SOD, GSH-PX in serum were detected after the experiment. The contents of LF, LPO and MAO-B activity in brain tissue were detected by ELISA method; the ultrastructural changes of brain tissue were observed by transmission electron microscopy.RESULTS Compared with the normal group, rat subacute aging model was replicated successfully; high-dose and medium-dose of SDF can significantly increase the thymus and spleen index of rats. high-dose of SDF can significantly decrease the contents of MDA, PC, AGES and increase the activity of SOD, GSH-PX; high-dose and medium-dose of SDF can reduce the LF, LPO contents and MAO-B activity in brain tissue. In addition, high-dose and medium-dose of SDF can improve the degeneration of neurons in rat brain.CONCLUSION SDF has obvious anti-aging effect on aging rats, which may be related to the antioxidant and the influence of neurotransmitters.
FEI T, FEI J, HUANG F, et al. The anti-aging and anti-oxidation effects of tea water extract in Caenorhabditis elegans . Exp Gerontol, 2017, 97:89-96.
HARMAN D. Aging: a theory based on free radical and radiation chemistry. J Gerontol, 1956, 11(3):298-300.
FAN H Y, GU R S, REN K, et al. Effect of flavonoids from sophora flavescens in aging mice induced by D-galactos. China J Chin Mater Med(�й���ҩ��־), 2015, 40(21):4240-4244.
ZENG L B, ZHANG J P. Discussion on the pharmacology and clinical application of traditional Chinese medicine dragon blood. Asia-Pacific Tradit Med(��̫��ͳҽҩ), 2014, 10(3):43-44.
YANG H, WU Y, MA Y Y, et al. Study on compatibility of traditional Chinese medicine active constituents with antioxidant activity. China J Chin Mater Med(�й���ҩ��־), 2012, 37(12):1826-1829.
LI R, LUO S Y. Protective effects of Longxuetongluo capsule on cerebral ischemia injury in rats. Chin J Clin Pharmacol Ther(�й��ٴ�ҩ��ѧ������ѧ), 2017, 22(11):1232-1236.
XU S Y,BIAN L R,CHEN X. Pharmacological Experimental Methodology(ҩ�����鷽��ѧ) . Beijing: People��s Medical Publishing House, 2002:1552.
LI Y Q, YE S D, ZHAI L M, et al. Effects of metformin on expression of renal tissue AGEs in type 2 diabetic rats. Chin Pharmacol Bull(�й�ҩ��ѧͨ��), 2016, 32(5):703-707.
MAO Q J, CHEN B G. Effects of electroacupuncture on microvascular ultrastructure and VEGF expression of the right cerebral cortex in focal cerebral ischemia/reperfusion injury rats. Acupunct Res(����о�), 2012, 37(6):476-481.
XU Y, LI Y, MA L, et al. D-galactose induces premature senescence of lens epithelial cells by disturbing autophagy flux and mitochondrial functions. Toxicol Lett, 2018, 289:99-106.
FENG G, ZHENG X, YAN S, et al. Role of advanced glycation end products and nitric oxide in patients with diabetic peripheral neuropathy. Prog Mod Biomed(�ִ�����ҽѧ��չ), 2010, 10(14):2684-2686.
KONG L F, WEI Q W, FEDAIL J S, et al. Effects of thyroid hormones on the antioxidative status in the uterus of young adult rats. J Reprod Dev,2015, 61(3):219-227.
JEANNETTE K, CHRISTIANE O, MARTIN H, et al. Mitochondrial contribution to lipofuscin formation. Redox Biol, 2017, 11:673-681.
LUO S Y, DENG X F, LIU P, et al. Anti-aging effects of kangshuailing ointment and its mechanism. Chin J Clin Pharmacol Ther(�й��ٴ�ҩ��ѧ������ѧ), 2018, 23(1):24-28.