Abstract��OBJECTIVE The influence of different processing methods on the quality of Schisandrae Chinensis Fructus was investigated based on simultaneous determination of multiple bioactive constituents combined with multivariate statistical analysis.METHODS An analytical method based on UFLC-QTRAP-MS/MS was used for the simultaneous determination of fifteen components including lignans, such as schizantherin B, schisandrol B, schizandrin C, ��-schisandrin, deoxyschizandrin, schisantherin, schisandrin, schisanhenol, gomisin D, gomisin J, and angeloylgomisin H and organic acids, such as (S)-malic acid, D (-)-tartaric acid, protocatechuic acid, and quinic acid in Schisandrae Chinensis Fructus under different processing methods. Besides, clustering analysis and grey relation analysis(GRA) have been applied to comprehensively analyze and evaluate the results of different processing methods according to the content of 15 components.RESULTS The results showed that fifteen components had good linearity, and correlation coefficients were more than 0.999 1. The method exhibited good precision, repeatability and stability. The average recoveries were between 96.64% and 99.96%, and the relative standard deviations were less than 5%. In addition, GRA results indicated that the quality of oven drying samples were better than the quality of oven drying after steaming samples. The quality of S7 was the best, followed by S10.CONCLUSION The established method was accurate and reliable, which could be used to appraise the quality of Schisandrae Chinensis Fructus. Our study may lay the way for the processing method of Schisandrae Chinensis Fructus in optimization, normalization and standardization.
�����, ʯ��, ����˼, ��ѵ��, ����ï, ���÷, ��ӱ, �Ի�. ��ͬ�ӹ���������ζ����ľ֬�ؼ��л�����ɷֵ�Ӱ��[J]. �й�ҩѧ��־, 2018, 53(23): 1984-1991.
CHEN Shu-yu, SHI Jing-jing, ZOU Li-si, LIU Xun-hong, TANG Ren-mao, MA Ji-mei, YAN Ying, ZHAO Hui. Effects of Different Processing Methods on Lignans and Organic Acids in Schisandrae Chinensis Fructus. Chinese Pharmaceutical Journal, 2018, 53(23): 1984-1991.
LI Y Z, WANG Z, MA Z, et al. Effect of extract from the stems of Schisandra chinensis on high fat diet-induced obesity in mice. Chin Pharm J(�й�ҩѧ��־), 2018,53(7):518-525.
[3]
QI L, JIN H, ZHAO D H,et al. Effect of Schisandra Lignans on the apoptosis of SHG-44 glioma neurospheres. Chin Pharm J(�й�ҩѧ��־), 2014, 49(2):113-116.
[4]
LI X G, GAO Q, WENG W, et al. Research progress of fructus Schisandra chinesis (Turcz) Bail and its pharmacological action. J Chin Med Mater(��ҩ��), 2005, 28(2):156-159.
[5]
MING Y B, ZHAO H, ZHOU L, et al. Reseach progress of Schisandra chinesis (Turcz) Bail. Pract Pharm Clin Remed(ʵ��ҩ�����ٴ�), 2007,10(6):365-367.
[6]
SZOPA A, EKIERT R, EKIERT H. Current knowledge of Schisandra chinensis(Turcz.) Baill. (Chinese magnolia vine) as a medicinal plant species: a review on the bioactive components, pharmacological properties, analytical and biotechnological studies. Phytochem Rev, 2017,16(2):195-218.
[7]
LI L, XIAO Y Q, LI Y, et al. Main substance and biological activity of acidic components of Schisandra chinensis. Chin J Exp Tradit Med Form(�й�ʵ�鷽��ѧ��־), 2014,20 (6):70-73.
[8]
DUAN J A, SU S L, LV L J, et al. Traditional expreiencess and modern cognition on primary processing of traditonal Chinese medicinal materials. China J Chin Mater Med(�й���ҩ��־), 2009,34(24):3151-3157.
[9]
AN K L, LI D K, ZHOU D Z, et al. Effects of different drying methods on quality of Schisandra chinesis (Turcz) Baill. China J Chin Mater Med(�й���ҩ��־), 2014,39(15):2900-2906.
2018, Vol. 53 
