目的 采用一测多评法(QAMS)同时测定银杏叶片中4种内酯类成分的含量,并验证本方法在银杏叶片质量控制中的应用价值。方法 以白果内酯为参照物,建立银杏叶片中的白果内酯与银杏内酯A、B、C之间的相对校正因子;采用外标法测定白果内酯的含量,通过相对校正因子计算银杏内酯A、B、C的含量,以此计算各内酯成分的含量;并通过方法学验证与外标法测定的含量数据比较,验证一测多评法的准确度与可行性。结果 采用外标法和一测多评法对5批银杏叶片进行含量测定,4种内酯类成分的测定结果无显著差异,实验所得的校正因子重复性好。结论 建立的校正因子重复性良好,可用于银杏叶片中多个成分的定量分析与质量评价。
Abstract
OBJECTIVE To determine simultaneously four lactones ingredients content in Yinxingye tablets by quantitative analysis of multi-components by single-marker (QAMS), and to verify the application value of this method in the quality control of Yinxingye tablets. METHODS With Ginkgo esters as the indexes,the relative correction factor(RCFs) between bilobalide and ginkgolides A,B,C were established. The content of bilobalide in Yinxingye tablets were determined by external standard method,the content of ginkgolides A,B,C in Yinxingye tablets was calculated by the relative correction factor. The external standard method and QAMS determined contents of 4 lactones, Rationality, feasibility and repeatability of the QAMS method were verified by comparing the results obtained from two different methods. RESULTS There was no significant differences between the calculated value and estimated value on QAMS and external standard method of the 5 batches of Yinxingye tablets,the results showed that the RCF was authentic. CONCLUSION The established correction factor is good repeatability,and could be used for quantitative analysis and quality evaluation of multiple components in Yinxingye tablets.
关键词
一测多评法 /
银杏叶片 /
白果内酯与银杏内酯 /
相对校正因子
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Key words
multi-components by single-marker (QAMS) /
Yinxingye tablet /
total lactones /
relative correction factor(RCFs)
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中图分类号：
R969
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参考文献
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