Spectrum and Toxicity Relationships between Multi-Wavelength HPLC Fingerprints and Acute Toxicity of Different Extracts of Aconitum sinomontanum Nakai
LI Yun1, MIAO Xiao-lou3, ZHANG Li-jun1, MA Dong-ni1, YANG Xiu-juan1, DAI Hai-rong1, YANG Zhi-jun1,2*, FAN Qin1,2, MA Jun1,2
1. Department of Pharmacy, Gansu University of Traditional Chinese Medicines, Lanzhou 730000, China; 2. Provincial-Level Key Laboratory for Tibet Herbal Chemicals and Quality Research in Gansu Colleges and Universities, Lanzhou 730000, China; 3.Key Laboratory of New Animal Drug Project, Gansu Province, Key Laboratory of Veterinary Pharmaceutics Discovery, Ministry of Agriculture, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS, Lanzhou 730050, China
Abstract��OBJECTIVE To establish the HPLC multiple wavelength chromatographic fingerprints (MWCF) of different extracts of Aconitum sinomontanum Nakai (ASN) to clarify the attribution of the fingerprint peaks and their contribution to the acute toxicity. METHODS The experimental drugs (extracts of petroleum ether, chloroform, ethyl acetate, butanol, alcohol and water) were obtained by means of systematic solvent extraction from the 95% ethanol extract of ASN. The HPLC MWCF of different extracts of ASN were established by mean fingerprint method (MFM). The acute toxicity of different extracts in mice were carried out by measuring the median lethal dose (LD50) and maximum dose. The relationship between spectrum and toxicity was established by gray relational analysis. RESULTS The MWCF of different extracts of ASN were established. The acute toxicity of ASN was caused not only by lappacontine (LAP) and ranaconitine, the contribution of other diterpenoid alkaloids should not be neglected, and the contribution of different peaks to toxicity was ranked as (51, 38, 37, 35, 20) ��34��39��32��31��33. CONCLUSION The MWCF developed by MFM can maximally retain the fingerprint peaks, achieve fingerprint information maximization, and effectively improve fingerprint signal quality, thus providing a reference for the comprehensive quality evaluation of ASN. The relationship between the MWCF of different extracts and the acute toxicity is paralleled to some extent. And this will lay a foundation for the research of the toxicity mechanism of ASN.
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LI Yun, MIAO Xiao-lou, ZHANG Li-jun, MA Dong-ni, YANG Xiu-juan, DAI Hai-rong, YANG Zhi-jun, FAN Qin, MA Jun. Spectrum and Toxicity Relationships between Multi-Wavelength HPLC Fingerprints and Acute Toxicity of Different Extracts of Aconitum sinomontanum Nakai. Chinese Pharmaceutical Journal, 2018, 53(22): 1936-1943.
Editorial Board of the Chinese Herbal. Chinese Materia Medica Volume V�� (���л����ݡ����߾�) [M]. Shanghai:Shanghai Science and Technology Publishers, 1999: 1746-1747.
[2]
Jiangsu College of New Medicine. Dictionary of Medicinal Plant(��ҩ��ǵ�) [M]. Shanghai:Shanghai Science and Technology Publishers, 2001: 751-752.
[3]
Food and Drug Administration. Chinese Herbal Medicine Standards of Gansu Province(����ʡ��ҩ�ı�) [M]. Lanzhou:Gansu Culture Publishers, 2009: 117-118.
[4]
WANG Y Z, XIAO Y Q, ZHANG C, et al. Study of analgesic and anti-inflammatory effects of Lappaconitine gelata[J]. J Trad Chin Med, 2009, 29(2):141-145.
[5]
Tsinghua University. Multi-wavelength chromatographic fingerprint and its construction and method of application: China, CN1544935 [P]. 2004-11-10.
[6]
ZHANG L J, DAI H R, YANG Z J, et al. Study on HPLC fingerprint and quality control of analgesic and anti-inflammatory effect parts in crude and processed the roots of Aconitum sinomontanum Nakai[J]. J Chin Med Mater(�в�ҩ), 2017, 48(12):2442-2447.
[7]
SUN G X, CHE L, LI Y F. Mean fingerprint method for parallel multiple wavelength chromatographic fingerprints[J]. Cent South Pharm(����ҩѧ), 2011, 9(7):533-538.
[8]
SUN G X, WU B, BI K S. Quantitative identification Qijudihuang Pill by integrating overall information method based on parallel five wavelength high performance liquid chromatographic fingerprints[J]. Chin J Chromatogr(ɫ��), 2010, 28(9):877-884.
[9]
XU S Y, BIAN R L, CHEN X. Pharmacology Experimental Methodology[M]. Beijing: People 's Medical Publishing House, 2002: 885-888.
[10]
LIANG Q X, WANG H, XIAO Q Q, et al. Evaluation and calculation of bliss method for the median lethal dose (LD50)[J]. J Mathemat Med, 1995, 8(4):318-320.
[11]
DENG J L. The Essential Method of Gray System(��ɫϵͳ�Ļ�������)[M]. Wuhan: Huazhong University of Technology Press, 1987: 33-34.
[12]
YU P, LI K. Grey correlation analysis of data on Microsoft Excel[J]. Microcomputer Applications(�͵���Ӧ��), 2011, 27(3):29-31.
[13]
ZHANG L J, DAI H R, FAN Q, et al. HPLC Fingerprints and quality control of processed Aconitum sinomontanum Nakai[J]. Chin Pharm J(�й�ҩѧ��־), 2017, 52(22):2035-2041.
[14]
WEI H B, FAN A L, QIN Z P, et al. Two cases of ototoxic adverse reactions caused by inject Lappaconitine hydrobromide[J]. Chin J Hosp Pharm(�й�ҽԺҩѧ��־), 2012, 32(17):1413-1414.
[15]
WANG Y, WANG S, LIU Y, et al. Characterization of metabolites and cytochrome P450 isoforms involved in the microsomal metabolism of aconitine[J]. J Chromatogr B, 2006, 844(2):292-300.
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