Abstract��OBJECTIVE To construct a network of active components-effect target of xiangdan injection and the network of protein interaction,to analyze the functions and pathways involved in the targets,and to investigate its mechanism of multicomponent-multitarget-multipathway in the treatment of coronary heart disease. METHODS The main active components in xiangdan injection, such as danshensu, salvianolic acid A, salvianolic acid B, salvianolic acid D, tanshinone ��A, protocatechuic aldehyde,rosmarinic acid,and oxidated nerolidol were used as the research object to predict targets of active components in xiangdan injection on the basis of reverse docking server (PharmMapper),to screen the targets for the treatment of coronary heart disease through the human genome annotation database (GeneCards), to construct active ingredients-target disease network using Cytoscape 3.5.1 software, to construct target protein protein interaction(PPI) network using STRING platform,and to analyze the target GO biological process and KEGG metabolic pathway using biological information annotation database (DAVID). RESULTS Eight pharmacodynamic components of xiangdan injection act on ACE, HSP90AA1, AKT1, NOS3, F2, F7 and other 257 potential targets for coronary heart disease,which mainly related to the 13 signaling pathways and 12 biological processes. CONCLUSION The main active ingredient of xiangdan injection could treat the coronary heart disease by PPAR, complement and coagulation cascades, HIF-1, estrogen signaling pathway, play a role of anti-inflammatory, anticoagulant, anti-hypoxia, anti-apoptosis and regulating hormones.
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LI Yue-wen, LIU Zhi-qiang, ZHAO Yue, CHEN Chun-lin, WANG Bo-long. Network Analysis of Main Active Ingredients-Target-Pathway of Xiangdan Injection in Treatment of Coronary Heart Disease. Chinese Pharmaceutical Journal, 2018, 53(22): 1927-1935.
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2018, Vol. 53 
