目的 观察野生冬虫夏草和冬虫夏草人工繁育品对肾阳虚小鼠的补肾作用,并比较野生及人工繁育冬虫夏草药理作用的差异。方法 建立氢化可的松诱导的肾阳虚小鼠模型,灌胃给予2种冬虫夏草(低、高剂量),观察各组小鼠体重、负重游泳能力、性腺器官及免疫器官脏器指数、肾脏和睾丸组织形态等指标。结果 野生冬虫夏草和冬虫夏草人工繁育品可改善氢化可的松所致肾阳虚小鼠体重增加变慢,负重游泳时间缩短,机体抗氧化能力下降,胸腺、脾、储精囊、包皮腺等腺体指数下降,肾脏和睾丸组织形态出现病理性改变的现象。结论 野生冬虫夏草和冬虫夏草人工繁育品均具有显著的逆转氢化可所致小鼠肾阳虚的活性,具有一定的临床应用指导意义。
Abstract
OBJECTIVE To observe the renal protective effect of cultivated and wild Cordyceps sinensis on the the model mice of kidney-Yang deficiency, and to compare the differences of pharmacological effects between cultivated and wild Cordyceps sinensis. METHODS The model mice of kidney-Yang deficiency was induced by hydrocortisone in male mice which were randomly divided into seven groups: high dose group of cultivated Cordyceps sinensis, low dose of cultivated Cordyceps sinensis, high dose group of wild Cordyceps sinensis, low dose group of wild Cordyceps sinensis, control group, model group and positive drug group. The seven groups'index of physical status, swimming duration, gonad organ and immune organ, renal and testicular histology were observed. RESULTS Both cultivated and wild Cordyceps sinensis increased the body weight, prolonged the swimming time, improved sexual organ and immune organ index, ameliorated the pathological changes in renal and testicular histology, and enhanced the antioxidant ability of the model mice of kidney-Yang deficiency. There was no obvious difference in renal protective effect between cultivated and wild Cordyceps sinensis. CONCLUSION Both cultivated and wild Cordyceps sinensis can improve the kidney-Yang deficiency in male mice induced by hydrocortisone. Cultivated Cordyceps sinensis can be used as the alternative choice of wild Cordyceps sinensis in clinic.
关键词
野生冬虫夏草 /
肾阳虚 /
补肾作用 /
人工繁育冬虫夏草
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Key words
wild Cordyceps sinensis /
kidney-Yang deficiency /
renal protective effect /
cultivated Cordyceps sinensis
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中图分类号：
R963
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参考文献
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脚注
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基金
中药饮片质量保障体系研究项目资助(201507002)
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