Abstract��OBJECTIVE To establish an HPLC method for simultaneous determination of 12 constituents in N��jin pills. METHODS The analysis was carried out on Agilent Eclipse plus C18 column(4.6 mm��250 mm,5 ��m), using a mobile phase of acetonitrile (A)-0.2%formic acid solution (B) by a gradient elution program (0-25 min, 10%-35% A; 25-37 min, 35%-59% A; 37-40 min, 59%-75% A) at a flow rate of 1 mL��min-1 at 30 ��. The detection wavelength was set at 230 nm in the first 20 min, and then changed to 270 nm between 20 and 40 min. RESULTS The linear ranges of paeoniflorin, liquiritin, ferulicacid, narirutin, aurantiamarin, baicalin, wogonoside, cinnam aldehyde, baicalein, paeonol, ammonium glycyrrhetate and wogonin fell within the ranges of 0.011-1.062 ��g, 0.002-0.237 ��g, 0.003-0.259 ��g, 0.006-0.641 ��g, 0.029-2.925 ��g, 0.033-3.339 ��g, 0.008-0.834 ��g, 0.003-0.258 ��g, 0.008-0.772 ��g, 0.008-0.824 ��g, 0.007-0.724 ��g and 0.005-0.539 ��g, respectively. The recoveries were 99.3%, 98.4%, 99.1%, 99.4%, 99.5%, 99.6%, 99.4%, 99.2%, 99.0%, 99.6%, 99.1%, and 99.4%, respectively. The relative standard deviations were 0.35%, 1.02%, 0.33%, 0.38%, 0.18%, 0.27%, 0.40%, 0.74%, 0.53%, 0.16%, 0.08% and 0.21%, respectively (n=6). CONCLUSION This method is simple, accurate and convenient for the quality control of N��jin pills.
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2018, Vol. 53 
