Anti-Aging Effects and Mechanism of Red Ginseng Extract on D-Galactose Induced Aging Mice
ZENG Lu-lu1, DING Chuan-bo1,2, YANG Shuo1, SHE Xin-xin1, LIU Xing-long1, LI Zi-qizng1, WU Qin-chao1, LIU Wen-cong1*, ZHENG Yi-nan1*
1. College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China; 2. Hunchun Huarui Ginseng Industry Bioengineering Co., Ltd., Hunchun 133300, China
Abstract��OBJECTIVE To investigate the protective mechanism of red ginseng extract(RG) on aging mice induced by D-galactose and the correlation between tumor necrosis factor-�� (TNF-��)��glutathione peroxidase 1 (GPX1), glutathione peroxidase 2 (GPX2), interleukin-1�� (IL-1��), interleukin-6 (IL-6) and interleukin-10 (IL-10). METHODS Aging mice models were caused by D-galactose and randomly divided into control group, model group, RG low, medium and high dose (100, 200, 400 mg��kg-1) groups. The preparation of model and drug delivery, after 42 d, the mice were killed and determination of spleen index; the activity of superoxide dismutase (SOD) and malondialdehyde (MDA) in serum and brain tissue were measured. In serum, the levels of acetylcholinesterase (AChE), catalase (CAT), glutathione peroxidase (GSH-PX), inducible nitric oxide synthase (i-NOS) were measured. Enzyme-linked immunosorbent assay (ELISA) was used to test the levels of cyclooxygenase-2 (COX-2), TNF-��, IL-1�� and IL-6 in brain tissue. Real time quantitative reverse transcription polymerase chain reaction (RT-PCR) was used to detect the correlation of TNF-��, GPX1, GPX2, IL-1��, IL-6 and IL-10 gene mRNA expression in brain tissue. RESULTS The RG can enhance the spleen index, increase the activity of SOD in serum and brain tissue and AChE, CAT and GSH-PX in serum, decrease the content of MDA in serum and brain tissue, and decrease the content of iNOS in serum and the content of COX-2, TNF-��, IL-1�� and IL-6 in brain tissue. At the same time, it significantly inhibited the mRNA expression of TNF-��, IL-1�� and IL-6 in the brain tissue of mice, and increased the expression of GPX1, GPX2 and IL-10 mRNA in the brain tissue, and had statistical significance. CONCLUSION Concentrated solution of red ginseng extract could significantly exhibit anti-aging effects, its mechanism may be related to the TNF-��, GPX1, GPX2, IL-1��, IL-6 and IL-10 expression in aging, enhancing immune function, and removing free radicals and inflammatory factor.
XIANG Y, CHEN L B, YAO H, et al. Protective mechanism of ginsenoside Rg1 on hippocampus of aging mice induced by D-galactose. Chin Tradit Herb Drugs(�в�ҩ), 2017, 48(18):3789-3795.
[3]
VOCES J, ALVAREZ A I, VILA L, et al. Effects of administration of the standardized Panax ginseng extract G115 on hepanic antioxidant function after exhaustive exercise. Comp Biochem Physiol, 1999, 123(2):175-184.
[4]
LEI X J, FENG K, SUN L W, et al. Research progress on anti-aging mechanism of Ginsenoside. Amino Acids Biotic Resources(��������������Դ), 2010, 32(1):44-47.
[5]
XU H X, JIANG J P, XU P, et al. Antioxidant activity of polysaccharide from radix ginseng rubra. J Zhejiang Univ Tradit Chin Med(�㽭��ҽҩ��ѧѧ��), 2011, 35(6):909-912.
[6]
ZHANG Y Z, SU G Y, XIA X Y, et al. Research progress in hypoglycemic effect of natural dammarane saponins. Chin Tradit Herb Drugs(�в�ҩ), 2016, 47(15):2758-2763.
[7]
LEE Y, OH S. Administration of red ginseng ameliorates memory decline in aged mice. J Ginseng Res, 2015, 39(3):250-256.
[8]
ZENG L L, ZHAO T, LI Y, et al. Simultaneous determination of four kinds of rare saponins in black ginseng by HPLC. Chin Pharm J(�й�ҩѧ��־), 2017, 52(12):1069-1072.
[9]
LIU Z F, LIU M H, SUN Q, et al. Anti-aging effect and mechanism of concentrated solution of aqueous extract form Penthorum chinense. Chin Tradit Herb Drugs(�в�ҩ), 2016, 47(13):2319-2323.
[10]
YU X, LI S, YANG D, et al. A novel strain of Lactobacillus mucosae isolated from a Gaotian villager improves in vitro and in vivo antioxidant as well as biological properties in D-galactose-induced aging mice. J Dairy Sci, 2016, 99(2):903-914.
[11]
JIN S L, YIN Y G. In vivo antioxidant activity of total flavonoids from indocalamus leaves in aging mice caused by D-galactose. Food Chem Toxicol, 2012, 50(10):3814-3818.
[12]
ZHANG Y, HAO Y, YANG, et al. Effects of different steaming processes on ginsenosides from Ginseng Radix et Rhizoma rubra. J Med Sci Yanbian Univ (�ӱߴ�ѧҽѧѧ��), 2009, 32(4):244-246.
[13]
GONG Y S, GUO J, HU K, et al. Ameliorative effect of lotus seedpod proanthocyanidins on cognitive impairment and brain aging induced by D-galactose. Exp Gerontol, 2016, 74:21-28.
[14]
TIAN J Z, YANG C Z, YANG H M, et al. Effect of compound rhubarb preparation on ChAT, AChE activity and Ach content in cerebral cortex and hippocampus of aged mice. Clin Gerontol(�й�����ѧ), 1999, 19(1):32-33.
[15]
CHEN Y B. To study the effect and mechanism of iNOS in high fat diet induced learning and memory deficits . Guangzhou:Southern Medical University, 2013.
[16]
HU Z, LIU D D. Effects of Calcium agent from cultured Cordyceps sinensis on anti-aging and immunoregulation in mice. Chin Gerontol(�й�����ѧ), 2010, 30(19):2780-2781.
[17]
RUAN Q, LIU F, GAN Z, et al. The anti-inflamm-aging and hepatoprotective effects of huperzine A in D-galactose-treated rats. Mech Ageing Dev, 2013,134(3-4):89-97.
[18]
HAN C H, LIN Y F, LIN Y S, et al. Effects of yam tuber protein, dioscorin, on attenuating oxidative status and learning dysfunction in D-galactose-induced BALB/C mice. Food Chem Toxicol, 2014,65(1):356-363.
[19]
LI C P, ZHANG M S, LIU J, et al. Study on mechanism of ginsenoside Rg1 delaying brain aging. China J Chin Mater Med(�й���ҩ��־), 2014, 39(22):4442-4447.
[20]
LEE Y, OH S. Administration of red ginseng ameliorates memory decline in aged mice. J Ginseng Res, 2015, 39(3):250-256.
[21]
SABA E, JEONG D H, ROH S S, et al. Black ginseng-enriched Chong-Myung-Tang extracts improve spatial learning behavior in rats and elicit anti-inflammatory effects in vitro. J Ginseng Res, 2017, 41(2):151-158.
2018, Vol. 53 
