目的 探讨红参提取物(RG)对D-半乳糖(D-gal)所诱导的亚急性衰老小鼠模型的保护作用及与肿瘤坏死因子(TNF)-α、谷胱甘肽过氧化物酶(GPX)1、GPX2、白细胞介素(IL)-1β、IL-6、IL-10的相关性。方法 以D-半乳糖制备亚急性衰老小鼠模型,随机分为对照组、模型组、RG低、中、高剂量组 (100, 200, 400 mg·kg-1)。造模同时给药42 d后,测定脾脏指数;比色法测定血清和脑组织中超氧化物歧化酶(SOD)和丙二醛(MDA)、血清中的乙酰胆碱酯酶(AChE)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-PX)、诱导型一氧化氮合成酶(i-NOS);ELISA法测定脑组织中的环氧合酶-2(COX-2)、TNF-α、IL-1β及IL-6的水平;实时荧光定量逆转录-聚合酶链反应(RT-PCR)检测脑组织中TNF-α、GPX1、GPX2、IL-1β、IL-6及IL-10 mRNA表达。结果 RG能增强衰老模型小鼠的脾脏指数,提高血清和脑组织中的SOD、血清中的AChE、CAT、GSH-PX活性,降低血清和脑组织中MDA,及血清中iNOS和脑组织中COX-2、TNF-α、IL-1β及IL-6的含量。同时明显抑制小鼠脑组织中TNF-α、IL-1β及IL-6的mRNA表达量,增加脑组织中GPX1、GPX2及IL-10的mRNA表达量,且具有统计学意义。结论 RG具有明显的抗衰老作用,其机制可能与TNF-α、GPX1、GPX2、IL-1β、IL-6及IL-10表达、增强机体免疫能力、清除自由基和炎症因子有关。
Abstract
OBJECTIVE To investigate the protective mechanism of red ginseng extract(RG) on aging mice induced by D-galactose and the correlation between tumor necrosis factor-α (TNF-α)、glutathione peroxidase 1 (GPX1), glutathione peroxidase 2 (GPX2), interleukin-1β (IL-1β), interleukin-6 (IL-6) and interleukin-10 (IL-10). METHODS Aging mice models were caused by D-galactose and randomly divided into control group, model group, RG low, medium and high dose (100, 200, 400 mg·kg-1) groups. The preparation of model and drug delivery, after 42 d, the mice were killed and determination of spleen index; the activity of superoxide dismutase (SOD) and malondialdehyde (MDA) in serum and brain tissue were measured. In serum, the levels of acetylcholinesterase (AChE), catalase (CAT), glutathione peroxidase (GSH-PX), inducible nitric oxide synthase (i-NOS) were measured. Enzyme-linked immunosorbent assay (ELISA) was used to test the levels of cyclooxygenase-2 (COX-2), TNF-α, IL-1β and IL-6 in brain tissue. Real time quantitative reverse transcription polymerase chain reaction (RT-PCR) was used to detect the correlation of TNF-α, GPX1, GPX2, IL-1β, IL-6 and IL-10 gene mRNA expression in brain tissue. RESULTS The RG can enhance the spleen index, increase the activity of SOD in serum and brain tissue and AChE, CAT and GSH-PX in serum, decrease the content of MDA in serum and brain tissue, and decrease the content of iNOS in serum and the content of COX-2, TNF-α, IL-1β and IL-6 in brain tissue. At the same time, it significantly inhibited the mRNA expression of TNF-α, IL-1β and IL-6 in the brain tissue of mice, and increased the expression of GPX1, GPX2 and IL-10 mRNA in the brain tissue, and had statistical significance. CONCLUSION Concentrated solution of red ginseng extract could significantly exhibit anti-aging effects, its mechanism may be related to the TNF-α, GPX1, GPX2, IL-1β, IL-6 and IL-10 expression in aging, enhancing immune function, and removing free radicals and inflammatory factor.
关键词
红参 /
抗衰老 /
肿瘤坏死因子-α /
谷胱甘肽过氧化物酶1 /
谷胱甘肽过氧化物酶2 /
白细胞介素-1β /
白细胞介素-6 /
白细胞介素-10
{{custom_keyword}} /
Key words
red ginseng /
anti-aging /
TNF-α /
GPX1 /
GPX2 /
IL-1β /
IL-6 /
IL-10
{{custom_keyword}} /
中图分类号：
R965
{{custom_clc.code}}
({{custom_clc.text}})
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] Ch.P(2015)VolⅡ(中国药典2015年版.一部). 2015:153.
[2] XIANG Y, CHEN L B, YAO H, et al. Protective mechanism of ginsenoside Rg1 on hippocampus of aging mice induced by D-galactose. Chin Tradit Herb Drugs(中草药), 2017, 48(18):3789-3795.
[3] VOCES J, ALVAREZ A I, VILA L, et al. Effects of administration of the standardized Panax ginseng extract G115 on hepanic antioxidant function after exhaustive exercise. Comp Biochem Physiol, 1999, 123(2):175-184.
[4] LEI X J, FENG K, SUN L W, et al. Research progress on anti-aging mechanism of Ginsenoside. Amino Acids Biotic Resources(氨基酸与生物资源), 2010, 32(1):44-47.
[5] XU H X, JIANG J P, XU P, et al. Antioxidant activity of polysaccharide from radix ginseng rubra. J Zhejiang Univ Tradit Chin Med(浙江中医药大学学报), 2011, 35(6):909-912.
[6] ZHANG Y Z, SU G Y, XIA X Y, et al. Research progress in hypoglycemic effect of natural dammarane saponins. Chin Tradit Herb Drugs(中草药), 2016, 47(15):2758-2763.
[7] LEE Y, OH S. Administration of red ginseng ameliorates memory decline in aged mice. J Ginseng Res, 2015, 39(3):250-256.
[8] ZENG L L, ZHAO T, LI Y, et al. Simultaneous determination of four kinds of rare saponins in black ginseng by HPLC. Chin Pharm J(中国药学杂志), 2017, 52(12):1069-1072.
[9] LIU Z F, LIU M H, SUN Q, et al. Anti-aging effect and mechanism of concentrated solution of aqueous extract form Penthorum chinense. Chin Tradit Herb Drugs(中草药), 2016, 47(13):2319-2323.
[10] YU X, LI S, YANG D, et al. A novel strain of Lactobacillus mucosae isolated from a Gaotian villager improves in vitro and in vivo antioxidant as well as biological properties in D-galactose-induced aging mice. J Dairy Sci, 2016, 99(2):903-914.
[11] JIN S L, YIN Y G. In vivo antioxidant activity of total flavonoids from indocalamus leaves in aging mice caused by D-galactose. Food Chem Toxicol, 2012, 50(10):3814-3818.
[12] ZHANG Y, HAO Y, YANG, et al. Effects of different steaming processes on ginsenosides from Ginseng Radix et Rhizoma rubra. J Med Sci Yanbian Univ (延边大学医学学报), 2009, 32(4):244-246.
[13] GONG Y S, GUO J, HU K, et al. Ameliorative effect of lotus seedpod proanthocyanidins on cognitive impairment and brain aging induced by D-galactose. Exp Gerontol, 2016, 74:21-28.
[14] TIAN J Z, YANG C Z, YANG H M, et al. Effect of compound rhubarb preparation on ChAT, AChE activity and Ach content in cerebral cortex and hippocampus of aged mice. Clin Gerontol(中国老年学), 1999, 19(1):32-33.
[15] CHEN Y B. To study the effect and mechanism of iNOS in high fat diet induced learning and memory deficits . Guangzhou:Southern Medical University, 2013.
[16] HU Z, LIU D D. Effects of Calcium agent from cultured Cordyceps sinensis on anti-aging and immunoregulation in mice. Chin Gerontol(中国老年学), 2010, 30(19):2780-2781.
[17] RUAN Q, LIU F, GAN Z, et al. The anti-inflamm-aging and hepatoprotective effects of huperzine A in D-galactose-treated rats. Mech Ageing Dev, 2013,134(3-4):89-97.
[18] HAN C H, LIN Y F, LIN Y S, et al. Effects of yam tuber protein, dioscorin, on attenuating oxidative status and learning dysfunction in D-galactose-induced BALB/C mice. Food Chem Toxicol, 2014,65(1):356-363.
[19] LI C P, ZHANG M S, LIU J, et al. Study on mechanism of ginsenoside Rg1 delaying brain aging. China J Chin Mater Med(中国中药杂志), 2014, 39(22):4442-4447.
[20] LEE Y, OH S. Administration of red ginseng ameliorates memory decline in aged mice. J Ginseng Res, 2015, 39(3):250-256.
[21] SABA E, JEONG D H, ROH S S, et al. Black ginseng-enriched Chong-Myung-Tang extracts improve spatial learning behavior in rats and elicit anti-inflammatory effects in vitro. J Ginseng Res, 2017, 41(2):151-158.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}
基金
吉林省自然科学基金项目资助(20160101017JC);吉林省教育厅项目资助(吉教科合字 第197 号);吉林省科技发展计划重点科技攻关项目(20170204023NY);长春市科技创新(医药健康产业发展)重大专项资助(17YJ013)
{{custom_fund}}
PDF(1519 KB)
