Preparation and Drug-Loading Property of Mesoporous Silica Nanoparticles as a Novel Nano-Delivery Vector
ZHAO Song-feng1, CHENG Liang-xing2, ZHANG Xiao1, SHI Xiu-qin1, ZHANG Xiao-jian1*
1. Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; 2. Editorial Department of Basic Cancer and Clinic, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China;
Abstract��OBJECTIVE To prepare mesoporous silica nanoparticles (MSNs) modified by targeted ingredients to improve the tumor cell lethality of antitumor drugs. METHODS MSNs were prepared by template-hot water method, and modified with amino group and polyethyleneimine. The nano-carriers were characterized by their morphology, particle size and infrared absorption. Meanwhile, the intracellular uptake and in vitro antitumor activity of MSNs were evaluated on human breast carcinoma cell line (MCF-7). RESUTLS Three kinds of nanoparticles, MSNs, MSNs-NH2 and MSNs-PEI were all spherical, with mean diameters of (65��19), (77��17) and (117��21) nm, respectively. Infrared spectrum and differential thermal analysis results indicated that the functional groups were linked onto the surface of MSNs, and slower drug release was observed for MSNs-NH2 and MSNs-PEI. Moreover, the cellular uptake of three nanoparticles were 2.05, 2.89, and 2.63 times higher than free doxorubicin, and the cytotoxicity activity against MCF-7 cells were 1.77, 2.21, and 2.19 times, respectively. CONCLUSION The preparation method can be used to prepare MSNs nano-carriers. MSNs-NH2 and MSNs-PEI have improved carrier property and antitumor activity.
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