目的 鉴定具有拟雌激素作用的菟丝子在大鼠血清和粪便中的代谢产物,为阐明其药效物质基础提供依据。方法 采用HPLC-ESI-MS/MS技术,通过一级质谱信息初步推测化合物的相对分子质量和分子式,结合部分标准品的保留时间、碎片离子信息、质谱裂解规律及参考文献数据对菟丝子拟雌激素活性部位进入血清和粪便中的化学成分进行结构表征。结果 在给予菟丝子拟雌激素活性部位后的大鼠含药血清中共鉴定出5个原型成分及4个代谢产物,在给药后的粪便中共鉴定出11个化学成分,包括5个原型成分和6个代谢产物。结论 通过血液和粪便之间的对比分析,推测出菟丝子化合物在体内的主要代谢途径,为菟丝子拟雌激素作用物质基础研究提供了参考。
Abstract
OBJECTIVE To identify the metabolites of Cuscuta chinensis in the serum and feces of rats with estrogenic estrogen, and to provide the basis for elucidating the pharmacological basis of its efficacy. METHODS Using the HPLC-ESI-MS/MS technique, the molecular weight and molecular formula of the compounds were preliminarily estimated by the first-order mass spectrometry, and the chemical constituents of serum and feces were characterized by the retention time of the standard, the fragment ion information, the fragmentation law of mass spectrometry and the reference data. RESULTS Five prototypic components and 4 metabolites were identified in the serum. A total of 11 chemical constituents were identified in the feces after administration, including 5 prototype components and 6 metabolites. CONCLUSION Through the comparative analysis between serum and feces, the main metabolic pathways of Cuscuta chinensis compound are deduced, which provides reference for the basic research on the estrogenic substance of Cuscuta chinensis.
关键词
菟丝子 /
雌激素作用 /
代谢产物 /
代谢途经分析 /
液相色谱-质谱联用
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Key words
Cuscuta chinensis /
estrogenic effect /
metabolite /
metabolic pathway analysis /
LC-MS
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中图分类号：
R284
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脚注
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基金
黑龙江省自然科学基金项目资助(ZD2017014);哈尔滨商业大学2017年研究生创新科研资金项目资助(YJSCX2017-444HSD)
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