Qualitative Comparison of Chemical Compositions in Tanreqing Injection and Tanreqing Capsules by UPLC/Q-TOF-MSE
ZHAO Song-feng1, ZHANG Xiao1, SHI Xiu-qin1, ZHANG Xiao-jian1*, XU Ying2*
1. Department of Pharmacy, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; 2. Shanghai R&D Center for Standardization of Chinese Medicines, Shanghai 201203, China
Abstract��OBJECTIVE To identify rapidly the chemical constituents in Tanreqing injection and Tanreqing capsules by ultra-performance liquid chromatography with quadrupole-time-of-flight mass spectrometry (UPLC/Q-TOF-MSE).METHODS The separation was performed on a Waters Acquity UPLC BEH C18 column (1.0 mm��100 mm, 1.7 ��m) with acetonitrile-0.1% formic acid as mobile phase in gradient elution. ESI ion source was employed in negative ion mode. The differences of chemical compositions between the two preparations and the sources of these compounds were illustrated based on their retention time, accurate mass measurements and the mass fragments by comparison with those in the literature or database and the reference standards.RESULTS A total of 111 compounds including 13 unknown components were identified or tentatively characterized. Among these compounds, 14 were derived from Scutellariae Radix (SR) intermediate, 36 were from Bear Bile Powder(BBP) intermediate, 7 were from Caprae Hircus Cornu(CHC) intermediate, 34 were from Lonicerae japonicae Flos(LJF) intermediate and 22 were from Forsythiae Fructus(FF) intermediate. Moreover, quinic acid and rutin were simultaneously detected in LJF and FF intermediates, 28 constituents were unambiguously confirmed by their reference standards. However, 71 compounds were observed both in injection and capsules, while 24 compounds were only found in Tanreqing injection and 16 compounds only in the capsules.CONCLUSION The differences of chemical constituents between Tanreqing injection and capsules are effectively characterized by UPLC/Q-TOF-MSE method, which will facilitate the quality control of the two preparations.
���ɷ�,����,ʦ����,������,��Ӣ. ���ڳ���ЧҺ��ɫ��-�߷ֱ���������������̵����ע��Һ�ͽ��һ�ѧ�ɷֵĶ��Բ���[J]. �й�ҩѧ��־, 2018, 53(9): 739-748.
ZHAO Song-feng, ZHANG Xiao, SHI Xiu-qin, ZHANG Xiao-jian, XU Ying. Qualitative Comparison of Chemical Compositions in Tanreqing Injection and Tanreqing Capsules by UPLC/Q-TOF-MSE. Chinese Pharmaceutical Journal, 2018, 53(9): 739-748.
LIU W J,SUN L,LI D Y. Tanreqing injection in treatment of acute pneumonia (Ayndrome of Phlegm-Heat Obstructing Lung)[J] . Chin J Exp Tradit Med Form (�й�ʵ�鷽��ѧ��־),2014,20(10):220-222.
[2]
ZHANG L L,LI Z,XU X Y,et al. Pharmacodynamic studies on Tanreqing capsule [J] . Chin J Exp Tradit Med Form (�й�ʵ�鷽��ѧ��־),2004,10(3):37-40.
[3]
ZHANG Z H,ZHONG P P,XU Y. Comparison of chemical compositions between Tanreqing capsule and Tanreqing injection by HPLC-ESI-MS/MS [J] . Chin J Exp Tradit Med Form (�й�ʵ�鷽��ѧ��־),2017,23(12):44-51.
[4]
ZHAO H,YAN Y,ZOU L S,et al. Analysis of difference of chemical constituents in Magnolia officinalis var. biloba Rehd. et Wils from different commercial specifications by LC-triple TOF MS/MS [J] . Chin Pharm J(�й�ҩѧ��־),2017,52(19):1720-1726.
[5]
LIU S Y,XUE D S,LI J H,et al. Assay of amino acid in Cornu caprae hircus extract [J] . Cent South Pharm(����ҩѧ),2014,12(3):271-274.
[6]
PIRAUD M, VIANEY-SABAN C, PETRITIS K, et al. ESI-MS/MS analysis of underivatised amino acids:a new tool for the diagnosis of inherited disorders of amino acid metabolism. Fragmentation study of 79 molecules of biological interest in positive and negative ionisation mode [J] . Rapid Commun Mass Spectrom,2003,17(12):1297-1311.
[7]
SUN H, LIU M, LIN Z, et al. Comprehensive identification of 125 multifarious constituents in Shuang-huang-lian powder injection by HPLC-DAD-ESI-IT-TOF-MS [J] . J Pharm Biomed Anal,2015,115:86-106.
[8]
JIA W, WANG C, WANG Y, et al. Qualitative and quantitative analysis of the major constituents in Chinese medical preparation Lianhua-Qingwen capsule by UPLC-DAD-QTOF-MS [J] . Sci World J,2015,2015:731765.
[9]
ZENG J X, MA Y B, LI R M, et al. Qualitative analysis of active constituents in Forsythiae Fructus water decoction by HPLC-TOF-MS [J] . J Chin Med Mater (��ҩ��),2013,36(4):581-583.
[10]
GUO Y P,LIN L G,WANG Y T. Chemistry and pharmacology of the herb pair Flos Lonicerae japonicae-Forsythiae fructus[J] . Chin Med,2015,10:16.
[11]
HE L,CHEN Y,LIANG Z,et al. A rapid and comprehensive quality assessing method of Yin-Qiao-Jie-Du tablets using UHPLC-QTOF-MS in combination with multivariate statistical analysis [J] . J Pharm Biomed Anal,2016,124(2):129-137.
[12]
LI Y J,CHEN J,LI Y,et al. Identification and quantification of free radical scavengers in the flower buds of Lonicera species by online HPLC-DPPH assay coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry [J] . Biomed Chromatogr,2012,26(4):449-457.
[13]
JIAN L H,HU Q,ZHONG J Q,et al. Determination of forsythoside E in Tanreqing injection by LC-MS/MS [J] . Chin J Pharm Anal (ҩ�������־),2013,33(3):435-438.
[14]
GAO W,YANG H,QI L W,et al. Umbiased metabolite profiling by liquid chromatography-quadrupole time-of-flight mass spectrometry and multivariate data analysis for herbal authentication: classification of seven Lonicera species flower buds [J] . J Chromatogr A,2012,1245:109-116.
[15]
ZHANG L Y,LI Y B,LI L X,et al. Chemical constituents of Lonicera japonica Thunb. by RRLC-Q-TOF/MS [J] . Centr South Pharm (����ҩѧ),2012,10(3):204-208.
[16]
GUO H,LIU A H,YE M,et al. Characterization of phenolic compounds in the fruits of Forsythia suspensa by high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry [J] . Rapid Commun Mass Spectrom,2007,21(5):715-729.
[17]
HAN J,YE M,GUO H,et al. Analysis of multiple constituents in a Chinese herbal preparation Shuang-Huang-Lian oral liquid by HPLC-DAD-ESI-MSn [J] . J Pharm Biomed Anal,2007,44(2):430-438.
[18]
LI H,YU Y,WANG Z,et al. Chemical profiling of Re-Du-Ning injection by ultra-performance liquid chromatography coupled with electrospray ionization tandem quadrupole time-of-flight mass spectrometry through the screening of diagnostic ions in MSE mode [J] . PLoS One,2015,10(4):e0121031.
[19]
QIAO X,LI R,SONG W,et al. A targeted strategy to analyze untargeted mass spectral data: rapid chemical profiling of Scutellaria baicalensis using ultra-high performance liquid chromatography coupled with hybrid quadrupole orbitrap mass spectrometry and key ion filtering [J] . J Chromatogr A,2016,1441:83-95.
[20]
QI L W,CHEN C Y,LI P. Structural characterization and identification of iridoid glycosides, saponins, phenolic acids and flavonoids in Flos Lonicerae Japonicae by a fast liquid chromatography method with diode-array detection and time-of-flight mass spectrometry [J] . Rapid Commun Mass Spectrom,2009,23(19):3227-3242.
[21]
SHI X Q,KAN Q C,XU Y. Research on constituent change of bear bile powder during production process of Tanreqing capsule by HPLC-MS/MS [J] . Chin Pharm J (�й�ҩѧ��־),2017,52(22):2023-2034.
[22]
SARAFIAN M H,LEWIS M R,PECHLIVANIS A,et al. Bile acid profiling and quantification in biofluids using ultra-performance liquid chromatography tandem mass spectrometry [J] . Anal Chem,2015,87(19):9662-9670.
[23]
WISHART D S,JEWISON T,GUO A C,et al. HMDB 3.0-The Human Metabolome Database in 2013 [DB/OL] . Nucleic Acids Res,2013,41(D1):D801-D807. https://doi.org/10.1093/nar/gks1065
[24]
KUCHARSKA A Z,FECKA I. Identification of iridoids in Edible Honeysuckle berries (Lonicera caerulea L. var. kamtschatica Sevast.) by UPLC-ESI-qTOF-MS/MS [J] . Molecules,2016,21(9):1157-1181.
[25]
BI D,CHAI X Y,SONG Y L,et al. Novel bile acids from bear bile powder and bile of geese [J] . Chem Pharm Bull,2009,57(5):528-531.
[26]
JIAN L H,MAO X H,WANG K,et al. Structure determination of three novel bile acids from bear bile powder [J] . Acta Pharm Sin (ҩѧѧ��),2013,48(8):1297-1300.
[27]
WANG L M. Mechanism of action and adverse reaction of Tanreqing injection [J] . Clin J Tradit Chin Med (��ҽҩ�ٴ���־),2015,27(1):110-111.
2018, Vol. 53 
