主成分分析法考察不同采收期草珊瑚中7个化学成分的含量

徐翔英,衷林清,彭琴,郑冬梅,刘乾松,潘馨,廖华军

中国药学杂志 ›› 2018, Vol. 53 ›› Issue (9) : 675-681.

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中国药学杂志 ›› 2018, Vol. 53 ›› Issue (9) : 675-681. DOI: 10.11669/cpj.2018.09.004
论著

主成分分析法考察不同采收期草珊瑚中7个化学成分的含量

  • 徐翔英,衷林清,彭琴,郑冬梅,刘乾松,潘馨*,廖华军*
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Principal Components Analysis in Determination of Seven Chemical Constituents in Different Parts and Collecting Time of Sarcandra glabra by HPLC

  • XU Xiang-ying, ZHONG Lin-qing, PENG Qin, ZHENG Dong-mei, LIU Qian-song, PAN Xin*, LIAO Hua-jun*
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文章历史 +

摘要

目的 建立HPLC同时测定不同采收期草珊瑚中新绿原酸、绿原酸、隐绿原酸、槲皮素-3-O-β-D-葡萄糖醛酸苷、异嗪皮啶、山柰酚-3-O-β-D-葡萄糖醛酸苷和迷迭香酸的含量。方法 采用北京慧德易SP ODS-AQ C18分析柱(4.6 mm×250 mm,5 μm),流动相为乙腈(A)-0.2%磷酸水溶液(B),梯度洗脱(0~10 min,5% A→15% A;11~18 min,15% A→25% A;18~25 min,25% A;25~40 min,25% A→40% A),流速1.0 mL·min-1,检测波长为344 nm,柱温35 ℃。结果 新绿原酸、绿原酸、隐绿原酸、槲皮素-3-O-β-D-葡萄糖醛酸苷、异嗪皮啶、山柰酚-3-O-β-D-葡萄糖醛酸苷和迷迭香酸分别在9.32~466.00、11.25~562.50、10.94~547.00、8.68~434.00、10.48~524.00、9.66~483.00和10.86~543.00 μg·mL-1内与峰面积呈现良好的线性关系(r=0.999 6~0.999 9),平均加样回收率(n=6)在99.0%~101.5%之间,RSD为0.85%~1.8%。主成分分析法综合评定得草珊瑚最佳采收月份为9、10、11月份。结论 该方法准确可靠,适用于草珊瑚中7个化学成分的含量测定,为草珊瑚的产地采收提供参考。

Abstract

OBJECTIVE To develop an HPLC method for simultaneous determination of seven active constituents including neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, quercetin-3-O-β-D-glucuronide, isofraxidin, kaempferol-3-O-β-D-glucuronide and rosmarinic acid in different parts and various collecting time of Sarcandra glabra.METHODS The DIKMA Diamonsil C18(4.6 mm×250 mm,5 μm) column was used. acetonitrile(A)-0.2%phosphoric acid(B) was used as the mobile phase with gradient elution(0-10 min,5% A→15% A;11-18 min,15% A→25% A;18-25 min,25% A;25-40 min,25% A→40% A),at a flow rate of 1.0 mL·min-1. The detection wavelength was set at 344 nm and the column temperature was maintained at 35 ℃.RESULTS The calibration curves of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, quercetin-3-O-β-D-glucuronide, isofraxidin, kaempferol-3-O-β-D-glucuronide and rosmarinic acid were in good linearity over 9.32-466.00, 11.25-562.50, 10.94-547.00, 8.68-434.00, 10.48-524.00, 9.66-483.00 and 10.86-543.00 μg·mL-1 (r=0.999 6-0.999 9), respectively. The average recoveries (n=6)were in the range of 99.0%-101.5% and RSD were in the range of 0.85%-1.8%. The optimal parts and collecting time of Sarcandra glabra are aerial parts and from September to November according to the comprehensive evaluation.CONCLUSION The method is precise and reliable, can be used to determin the content of seven chemical constituents in Sarcandra glabra and provide a reference for collecting in cultivate area.

关键词

草珊瑚 / 不同采收期 / 异嗪皮啶 / 迷迭香酸 / 高效液相色谱法

Key words

Sarcandra glabra / different collecting time / isofraxidin / rosmarinic acid / HPLC

引用本文

导出引用
徐翔英,衷林清,彭琴,郑冬梅,刘乾松,潘馨,廖华军. 主成分分析法考察不同采收期草珊瑚中7个化学成分的含量[J]. 中国药学杂志, 2018, 53(9): 675-681 https://doi.org/10.11669/cpj.2018.09.004
XU Xiang-ying, ZHONG Lin-qing, PENG Qin, ZHENG Dong-mei, LIU Qian-song, PAN Xin, LIAO Hua-jun. Principal Components Analysis in Determination of Seven Chemical Constituents in Different Parts and Collecting Time of Sarcandra glabra by HPLC[J]. Chinese Pharmaceutical Journal, 2018, 53(9): 675-681 https://doi.org/10.11669/cpj.2018.09.004
中图分类号: R917   

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基金

国家中医药管理局中医药行业科研专项资助(201507002-01-03)
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