Synergetic Antibacterial Effect of Ultrasound Microbubbles Combined with Chitosan- Modified Polymyxin B Liposomes on Biofilm-producing Acinetobacter baumannii
FU Yu-ying1, YU Xian1*, LI Pan2, CAO Yang2, LIU Cheng-wei3, HU Bang-qin4
1. Department of Pharmacy, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China; 2. Chongqing Key Laboratory of Ultrasound Molecular Imaging, Chongqing 400010, China; 3. State Key Laboratory of Infectious Diseases and Parasites, Chongqing 400010, China; 4. Pharmacy College of Chongqing Medical University, Chongqing 400010, China
Abstract��OBJECTIVE To investigate the synergetic antibacterial effects of chitosan-modified polymyxin B liposomes(c-PMB-Lip) combined with ultrasound microbubbles on biofilm-producing Acinetobacter baumannii(AB) in vitro. METHODS c-PMB-Lip was prepared by an injection method. The minimum biofilm inhibition concentrations (MBIC) of the AB clinical isolates treated with different preparations of polymyxin B were determined. The antibacterial effects and the dose-effect relationship of chitosan-modified polymyxin B liposome group (c-PMB-Lip), ultrasound microbubble + polymyxin B group (USMB+PMB) and ultrasound microbubble + chitosan modified polymyxin B liposome group (USMB+c-PMB-Lip) were further revealed. RESULTS The MBIC of c-PMB-Lip was (8.0��2.0)��g��mL-1 against biofilm-producing AB. USMB could enhance the inhibitory effects of polymyxin B on biofilm-producing AB. Moreover, USMB combined with lipid agents could obtain the most significant inhibitory effects and even completely remove the biofilm-producing AB. With the drug concentration increasing, the antibacterial effects of each group showed a dose-effect relationship within a certain range. Compared with the sterile blank group, USMB+c-PMB-Lip could completely remove the bacterial biofilm and achieve a maximum antibacterial effects at 2 ��g��mL-1(P>0.05). CONCLUSION c-PMB-Lip combined with USMB have significant synergistic antibacterial effect on biofilm-producing AB.
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FU Yu-ying, YU Xian, LI Pan, CAO Yang, LIU Cheng-wei, HU Bang-qin. Synergetic Antibacterial Effect of Ultrasound Microbubbles Combined with Chitosan- Modified Polymyxin B Liposomes on Biofilm-producing Acinetobacter baumannii. Chinese Pharmaceutical Journal, 2018, 53(4): 283-289.
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2018, Vol. 53 
