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Effects and Underlying Mechanism of Extract of Scutellaria baicalensis Georgi Against LPS-Induced Inflammation in BV2 Cells
YANG Huana, YANG Yin-lina, CHENG Xiaoa, DENG An-junb, QIN Hai-linb, WANG Yue-huaa*, DU Guan-huaa*
Beijing Key Laboratory of Drug Target Identification and New Drug Screening; b. Laboratory of Natural Medicinal Chemistry, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China
Abstract��OBJECTIVE To investigate the anti-inflammatory effects of extract of Scutellaria baicalensis Georgi (SGE) and underlying mechanism by using LPS-induced microglial BV2 cells. METHODS MTT assay was used to observe the cell viability. The content of NO in cell supernatant was measured by Griess reagent. The levels of IL-1��, IL-6 and TNF-�� were detected by ELISA kits. The intracellular TLR4 expression was assayed by Western blotting. RESULTS The levels of NO, IL-1��, IL-6 and TNF-�� were significantly increased induced by LPS in the supernatant of BV2 cells (all P<0.01). However, co-treatment with SGE 100 ��g��mL-1 significantly decreased the production of related inflammatory factors including NO (P<0.01), IL-1��(P<0.01), IL-6 (P<0.01) and TNF-�� (P<0.05). Furthermore, SGE significantly inhibited the TLR4 expression induced by LPS in BV2 cells. CONCLUSION SGE is able to alleviate LPS-induced inflammatory responses in BV2 cells through down-regulation of TLR4 protein expression suggesting that SGE has therapeutic potential for the treatment of neuroinflammatory diseases.
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YANG Huan, YANG Yin-lin, CHENG Xiao, DENG An-jun, QIN Hai-lin, WANG Yue-hua, DU Guan-hua. Effects and Underlying Mechanism of Extract of Scutellaria baicalensis Georgi Against LPS-Induced Inflammation in BV2 Cells. Chinese Pharmaceutical Journal, 2018, 53(4): 268-272.
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2018, Vol. 53 
