目的 对美洲大蠊(Periplaneta americana)虫体体积分数95%乙醇提取物进行化学成分的分离和鉴定。方法 采用硅胶柱色谱,凝胶柱色谱,开放ODS柱色谱和半制备高效液相色谱等分离手段,对乙酸乙酯部位进行分离纯化,并利用核磁共振、质谱等现代波谱技术对化合物进行结构鉴定。结果 分离并鉴定出14个化合物,分别为水杨酸 (1),苯甲酸 (2),苯乙酸 (3),对羟基苯乙酸甲酯 (4),对羟基苯甲酸 (5),染料木素 (6),间羟基苯甲酸 (7),2-羟基马尿酸甲酯 (8),2,5-二羟基苯甲酸 (9),2-羟基马尿酸 (10),尿嘧啶 (11),环(缬氨酸-丙氨酸)二肽(12),N-乙酰多巴胺 (13)和环(L-脯氨酸-L-酪氨酸)二肽(14)。结论 化合物2,4,7~10, 12和14为首次从大蠊属昆虫中分离得到。
Abstract
OBJECTIVE To study the chemical constituents from Periplaneta americana. METHODS The 95% enthanol extract was isolated and purified by column chromatography on silica gel, Sephadex LH-20, ODS and semi-PHPLC. All compounds were identified by chemical and spectral analyses. RESULTS Fourteen compounds were isolated and purified from Periplaneta americana. Their structures were elucidated as salicylic acid (1), benzoic acid (2), phenylacetic acid (3), methyl 4-hydroxyphenylacetate (4), p-hydroxybenzoic acid (5), genistein (6), 3-hydroxybenzoic acid (7), methyl N-(2-hydroxybenzoyl) glycinate (8), 2,5-dihydroxybenzoic acid (9), (2-hydroxybenzoyl) glycine (10), uracil (11), cyclo(Val-Ala) (12), N-acetyldopamine (13), and cyclo(L-Pro-L-Tyr) (14), respectively. CONCLUSION Compounds 2, 4, 7-10, 12 and 14 are obtained from this genus for the first time.
关键词
美洲大蠊 /
环(缬氨酸-丙氨酸) /
环(L-脯氨酸-L-酪氨酸) /
化学成分 /
结构鉴定
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Key words
Periplaneta americana /
cyclo(Val-Ala) /
cyclo(L-Pro-L-Tyr) /
chemical constituent /
structure elucidation
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中图分类号：
R284
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脚注
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基金
中国医学科学院医学与健康科技创新工程(2016-I2M-3-015)
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