Abstract��OBJECTIVE To study the preparing METHODS of hispidulin liposomes and evaluate their antitumor effects in vitro. METHODS Hispidulin liposomes were prepared by thin film dispersion method, and the formulation was further optimized. At the same time, the liposomes particle size and Zeta potential were characterized separately. The antiproliferative effect of the liposomes on A549 cells was investigated by sulforhodamine B(SRB) method. RESULTS According to the optimal prescription, the encapsulation efficiency of hispidulin liposomes was (87.06��0.67)%. Additionally, the average particle size was (104.83��1.40) nm and the Zeta potential was (-4.61��0.32) mV. Furthermore, the hispidulin liposomes exhibited the strongest inhibiting effect on A549 cells in vitro with comparison with control groups. CONCLUSION The preparation method builtin our research is easy. And the liposomes are well distributed with high encapsulation efficiency. Meanwhile, the hispidulin liposomes exhibit the most significant antitumor effects in vitro.
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LI Xia, YING Xue, YAN He-lu, WANG Ya-hua, XU Hao-lun, LI Zhao-ping. Preparation of Hispidulin Liposomes and Its Antitumor Effect in Vitro. Chinese Pharmaceutical Journal, 2017, 52(18): 1624-1628.
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2017, Vol. 52 
