Inhibitory Effect of Ke-ai-xin Pill on Tumor Growth of Transplanted Colon Cancer Cell Line HCT116 in Nude Mice
LI Hua-yun2, LI Qin2, LIU Ming-hua3, SUN Qing1,3*
1. Drug Research Center, Affiliated Chinese Medicine Hospital of the Southwest Medical University, Luzhou 646000, China; 2. The Affiliated Hospital of the Southwest Medical University, Luzhou 646000, China; 3. School of Pharmacy, Southwest Medical University, Luzhou 646000, China
Abstract��OBJECTIVE To study the inhibitory effect of Ke-ai-xin pill on tumor growth and angiogenesis of transplanted colon cancer cell line HCT116 in nude mice. METHODS To establish the transplant tumor model in nude mice for HCT116. All tumor-bearing nude mice were randomly divided into control group (normal saline), positive group (bevacizumab,5 mg��kg-1, intraperitioneally), and low-, medium-, high-dose Ke-ai-xin pill-treated groups(200, 400 and 800 mg��kg- 1, intragastrically). The tumor xenografts were harvested and measured for their weights. Immunohistochemistry was used to detecte microvessel density (MVD)and vascular endothelial growth factor(VEGF)expression. RESULTS Ke-ai-xin pill inhibited tumor growth in nude mice in varying degrees. After the administration of each treatment group, TV, RTV, and T/C decreased significantly(P<0.05). SABC assay showed that MVD of Ke-ai-xin pill treatment group was sparse, MVD value decreased from (59.62��18.83) to (55.56��12.63),(46.72��11.57) and (41.35��10.27), Ke-ai-xin pill treatment group can significantly reduce the positive expression of VEGF. the positive expression rate reduced from(78.46��16.53)% to (58.64��14.19)%, (49.27��13.29)% and (43.68��11.71)%. CONCLUSION Ke-ai-xin pill can inhibit tumor growth of transplanted colon cancer cell line HCT116 in nude mice in varying degrees, reduce MVD value and the expression of VEGF in tumor tissue, so its antitumor activity may be related to inhibiting angiogenesis.
���,����,������,����. �˰����������᳦��ϸ��HCT116��ֲ����������������[J]. �й�ҩѧ��־, 2017, 52(18): 1609-1613.
LI Hua-yun, LI Qin, LIU Ming-hua, SUN Qing. Inhibitory Effect of Ke-ai-xin Pill on Tumor Growth of Transplanted Colon Cancer Cell Line HCT116 in Nude Mice. Chinese Pharmaceutical Journal, 2017, 52(18): 1609-1613.
GAO S Y, WU J, YANG G L. A study on correlation of tumor-associated macrophages infiltration,MMP-2 expression and angiogenesis in colon carcinoma[J]. Chin J Immunol(�й�����ѧ��־), 2016, 32(3):336-340.
[2]
JANG J H, KIM S K, CHOI J E, et al. Endothelial progenitor cell differentiation using cryopreserved, umbilical cord blood-derived mononuclear cells[J]. Acta Pharmacol Sin(�й�ҩ��ѧ��), 2007, 28(3):367-374.
[3]
XIAO H X, LIU M H, ZHONG L, et al. Effects of keaixin on the level of IL-2, IL-6, IL-12 and TNF-A in tumor-bearing mice[J]. Pharmacol Clin Chin Mater Med(��ҩҩ�����ٴ�), 2008, 24(2):82-84.
[4]
LIU M H, XIAO H S, REN M P, et al. Effects of keaixin and its components on the tumor growth and level of cytokine in tumor-bearing mice[J]. Lishizhen Med Mater Med Res(ʱ���ҽ��ҩ), 2010, 21(12):3150-3151.
[5]
ZHONG L, LIU M H, XIAO H S, et al. Effects of keaixin on tumor growth and immune function in tumor-bearing mice[J]. Chin J New Drugs(�й���ҩ��־), 2008, 17(23):2027-2030.
[6]
WEI W, WU X M, LI Y J. Experimental Methodology of Phamacology(ҩ��ʵ�鷽��ѧ)[M]. 4th ed. Beijing:People��s Medical Publishing House, 2010:1757-1779.
[7]
LIANG G, ZHANG D, ZHANG H, et al. Periplaneta Amaricana polypeptide inhibits tumor grouth in nude mice bearing human hepatocellular carcinoma Bel-7402 cells[J]. Chin J New Drugs(�й���ҩ��־), 2016,25(6):687-691.
[8]
LIU M H, YAO J, LI R, et al. Apoptosis of colon cancer cell line HCT116 induced by flavone components from Spina Gleditsiae[J]. Cancer Res Prevent Treat(���������о�),2011, 38 (6):643-646.
[9]
CHEN H X, LIU M H, LI M, et al. Antitumor activity in vitro of crude extract and pure flavone components from Spina Gleditsiae[J]. Lishizhen Med Mater Med Res(ʱ���ҽ��ҩ), 2013, 24(1):101-102.
[10]
WANG G Q. Quanguo Zhongcaoyao Huibian(Vol 2)(ȫ���в�ҩ���.���)[M]. Beijing:People��s Medical Publishing House, 2014:336-337.
[11]
LI R, ZHANG X, REN M P, et al. Effect of total saponin of Aralia on tumor grouth and immunological in S180 tumor-bearing mice[J]. J Chongqing Med Univ(����ҽ�ƴ�ѧѧ��), 2011, 36(2):148-151.
[12]
WU H B, TIAN Y, SUN Z H, et al. Structural modification and biological evaluation of monomeric compounds from Aralia elata[J]. Chin Tradit Herb Drugs(�в�ҩ),2016, 47(4):559-565.
[13]
REN M P, LIU M H, LI R, et al. Antitum or activity of Astragalus Polysaccharides[J]. Chin J New Drugs(�й���ҩ��־), 2010, 19(3):221-224.
[14]
LI Z Y, YANG G L, YAN C H, et al. Research progress on application of Astragalus Polysaccharides [J]. Liaoning J Tradit Chin Med(������ҽ��־), 2016, 43(7):1153-1155.
[15]
JEMAL A, BRAY F, CENTER M M, et al. Global cancer statistics[J]. CA Cancer J Clin, 2011, 61:69-90.
[16]
WANG Y, ZHANG J, LIU B. Influence mechanism of VEGF on colorectal cancer cells grouth[J]. Pract J Cancer(ʵ�ð�֢��־), 2016, 31(5):737-739.
[17]
ELKADY A I, HUSSEIN R A, EL-ASSOULI S M. Harmal extract induces apoptosis of HCT116 human colon cancer cells, mediated by inhibition of nuclear factor-��B and activator protein-1 signaling pathways and induction of cytoprotective genes[J]. Asian Pac J Cancer Prev, 2016, 17(4):1947-1959.
[18]
LIANG Q L, CHEN X D, WANG S M, et al. Expression of vascular endothelial growth factor and metastin in colorectal carcinoma[J]. J South Med Univ(�Ϸ�ҽ�ƴ�ѧѧ��), 2007, 27(10):1584-1587.
[19]
SAMUEL S, FAN F, DANG L H, et al. Intracrine vascular endothelial growth factor signaling in survival and chemo-resistance of human colorectal cancer cells[J]. Oncogene, 2011, 30(10):1205-1212.
[20]
SINGHAL S, VACHANI A, ANTIN-OZERKIS D, et al. Prognostic implications of cell cycle, apoptosis, and angiogenesis biomarkers in non-small cell lung cancer:a review [J]. Clin Cancer Res, 2005, 11(11):3974-3986.
2017, Vol. 52 
