Alkaloids from the Marine-Derived Fungus Aspergillus fumigatus YK-7 and Their Antitumor Activities
WANG Yu1, LI Zhan-lin2,3, BAI Jiao2,3, CHEN Zai-xing4, HUA Hui-ming2,3, LIU Tao1*
1. Department of Natural Products Chemistry, School of Pharmacy, China Medical University, Shenyang 110122, China; 2. Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China; 3. School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China; 4. Department of Central Laboratory, School of Pharmacy, China Medical University, Shenyang 110122, China
Abstract��OBJECTIVE To study the secondary metabolites of marine-derived fungus Aspergillus fumigatus YK-7. METHODS The compounds were isolated by several column chromatographic techniques, including silica gel, ODS, Sephadex LH-20 column chromatography, and HPLC, and their structures were identified on the basis of physicochemical properties and spectroscopic analysis. Trypan blue and MTT methods were applied for determining the effects of the compounds on proliferation of cancer cells in vitro. RESULTS Ten compounds were obtained, and their structures were identified as pseurotin A (1), pseurotin A1(2), 14-norpseurotin A (3), FD-838 (4), demethoxyfumitremorgin C (5), 9��-hydroxyverruculogen TR-2 (6), 6-methoxyspirotryprostatin B (7), spiro[5H,10H-dipyrrolo[1,2-a:1��,2��-d]pyrazine-2-(3H),2��-[2H]indole]-3��,5,10(1��H)-trione (8), terezine D (9), and 14-hydroxyterezine D (10). CONCLUSION Compounds 3, 6, 7, 9, and 10 are isolated from marine-derived fungus Aspergillus fumigatus for the first time. Compounds 1-4 exhibite moderate antiproliferative activity against selected cancer cell lines in vitro.
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WANG Yu, LI Zhan-lin, BAI Jiao, CHEN Zai-xing, HUA Hui-ming, LIU Tao. Alkaloids from the Marine-Derived Fungus Aspergillus fumigatus YK-7 and Their Antitumor Activities. Chinese Pharmaceutical Journal, 2017, 52(15): 1308-1312.
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