Simultaneous Determination of Furanocoumarins in Cultivated Changium smyrnioides Wollf and Its in Vitro Cultures by HPLC
CAI Jing1, CHEN Jian-wei1,2*, LI Xiang1,3, MA Yun1, HU Peng1, ZHANG Yuan-jiao1
1. School of Pharmacy,Nanjing University of Chinese Medicine, Nanjing 210023, China; 2. Jiangsu Key Laboratory for TCM Formulae Research, Nanjing 210023, China; 3. Jiangsu Key Laboratory for Chinese Material Medica Processing, Nanjing 210023, China
Abstract��OBJECTIVE To determine simultaneously the contents of xanthotoxin,bergaptol, and bergapten in cultivated Changium smyrnioides Wollf and its in vitro cultures by HPLC.METHODS Agilent-C18 column (4.6 mm��250 mm,5 ��m) was used for chromatographic separation and PAD was applied as detector. The flow rate was 1.0 mL��min-1with a mobile phase of methanol-water in gradient elution mode. The detection wavelength was set at 314 nm while the injection volume was 10 ��L.RESULTS The linear regression equations of xanthotoxin, bergaptol, and bergapten were Y=17 057��-87.689 (r=1.000 0), Y=23 828��-380.44 (r=0.999 9) and Y=37 123��-441.16(r=0.999 9), respectively. The contents of xanthotoxin and bergapten in cultivated and regenerated specimens were obviously higher than those in calli and suspension cells, while bergaptol was only detected in the latter two samples. A larger amount of furanocoumarins accumulated in the cells from leaves and petioles. CONCLUSION The established method is simple and effective with high sensitivity and good repeatability. It can be adopted in studies on the utilization of Changium smyrnioides, especially the regulation and induction of furanocoumarins.
�̾�, �½�ΰ, ����, ���S, ����, ����. ��ЧҺ��ɫ��ͬʱ�ⶨ������ԭֲ�����������������3����㶹�غ���[J]. �й�ҩѧ��־, 2017, 52(6): 494-499.
CAI Jing, CHEN Jian-wei, LI Xiang, MA Yun, HU Peng, ZHANG Yuan-jiao. Simultaneous Determination of Furanocoumarins in Cultivated Changium smyrnioides Wollf and Its in Vitro Cultures by HPLC. Chinese Pharmaceutical Journal, 2017, 52(6): 494-499.
Ministry of Environmental Protection of People's Republic of China, Institute of Botany of CAS.The Protection and Research of Rare and Endangered Plants(ϡ�б�Σֲ��ı������о�)[M]. Beijing:China Environmental Science Press, 1991:187.
CHANG J, GE Y, LU Y J, et al. A comparison of photosynthesis in endangered and non-endangered plants Changium smyrnioides and Anthriscus sylvestris[J].Photosynthetica, 2002, 40(3):445-447.
[4]
CHANG J, GUAN B H, GE Y, et al. Comparative studies on phenotypic plasticity of two herbs, Changium smyrnioides and Anthriscus sylvestris[J].J Zhejiang Univ Sci(�㽭��ѧѧ��), 2004, 5(6):656-662.
[5]
FU L G.China Plant Red Data Book(�й�ֲ���Ƥ��)[M]. Vol 1. Beijing:Science Press, 1991:532-535.
[6]
ZHANG P, CHEN J W, LI X.Determination of furocoumarin in the fruit of Changium smyrnioides by anti-HPLC[J]. J Tianjin Univ Tradit Chin Med (�����ҽҩ��ѧѧ��), 2007, 26(1):29-30.
[7]
ZHANG Y, CHEN J W, LI X, et al.Accumulation and distribution of furanocoumarins in rare medicinal plants Changium smyrnioides Wolff[J].Nat Prod Res Dev(��Ȼ�����о��뿪��), 2012, 24(4):510-513.
[8]
DUAN Z F, CHEN J W, LI X, et al. The comparison of contents of phellopterin in different parts of Changium smyrnioides Wolff[J].Chin Tradit Pat Med(�г�ҩ), 2008, 30(12):1851-1853.
[9]
GUY Y, CHEN J W, LI X, et al.Studies on chemical constituents of supercritical carbon dioxide extraction of the fruit of Changium smyrnioides Wolff[J].Chin Archives Tradit Chin Med(�л���ҽҩѧ��), 2010, 28(1):75-77.
[10]
BAIG G, YUAN F, MAOK J, et al.Chemical constituents in root bark of Changium smyrnioides[J].Chin Tradit Herb Drugs(�в�ҩ), 2014, 45(12):1673-1676.
[11]
BUYUKGUZEL E, HYRSL P, BUYUKGUZEL K. Eicosanoids mediate hemolymph oxidative and antioxidative response in larvae of Galleria mellonella L.[J].Comp Biochem Physiol A MolIntegr Physiol, 2010, 156(2):176-183.
[12]
WANG M, CHEN J W, LI X. Study on antitumor activity of five furanocoumarins fromthe root bark of Changium smyrnioides in vitro[J].Chin J Exp Tradit Med Form(�й�ʵ�鷽��ѧ��־), 2012, 18(6):203-205.
[13]
SHIKISHIMA Y, TAKAISHI Y, HONDA G, et al.Chemical constituents of Prangost sehimganica, structure elucidation and absolute configuration of courmarin and furanocoumarin derivatives with anti-HIV activity [J].Chem Pharm Bull, 2001, 49(7):877-880.
[14]
GU W, WUQ N, CHAOJ G, et al.Study on rapid propagation ofChangium smyrnioides [J].J Chin Med Mater(��ҩ��), 2008, 31(2):179-180.
[15]
ZHOU P, XIE C Q, CHEN J W, et al.Effects of browning inhibitors on suspension cells growth and secondary metabolites production in Changium smyrnioides [J].J Chin Med Mater(��ҩ��), 2015, 38(11):2250-2254.
[16]
DARIA H, ULRICH M.Biosynthesis of psoralens:psoralen 5-monooxygenase activity from elicitor-treated Ammi majus cells [J]. FEBS Lett, 1988, 239(2):263-265.
[17]
CHEN J W, LI X, WUL L, et al.Study on constituents of volatile oil of the tender stem and leaf of rare and precious Chinese medicinal plant Changium smyrnioides Wolff [J].Nat Prod Res Dev(��Ȼ�����о��뿪��), 2000, 12(3):48-51.
[18]
AYAL, YOSHIE K, YOSHIHIRO O, et al.Different responses of shoot and root cultures of Glahnia littoralis to yeast extract [J].J Nat Med, 2007, 61(1):30-37.
2017, Vol. 52 
