Simultaneous Determination of Six Bioactive Components of Jiawei Baiteng Extracts in Plasma by HPLC-TQ-MS/MS and Their Pharmacokinetics in Rats
WU Jie1,2, ZHU He1,2, WU Jun2, ZHONG Rong-ling2, JU Jian-ming1,2*
1. Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, China; 2. Jiangsu Branch of China Academy of Chinese Medical Sciences, Nanjing 210028, China
Abstract��OBJECTIVE To establish an HPLC-TQ-MS/MS assay for simultaneous determination of berberine, plantamajoside, saikosaponin a, tetrahydropalmatine, paeoniflorin and amygdalin in rat plasma and investigate the pharmacokinetics of Jiawei Baiteng extracts in rats. METHODS The separation was achieved on an Agilent Poroshell 120 EC-C18 column(3.0 mm��100 mm,2.7 ��m) at 35 ��. The mobile phase was consisted of 0.05% formic acid aqueous solution and acetonitrile containing 0.05% formic acid, eluting with a gradient procedure. Mass spectrometry was performed in the multiple reaction monitoring (MRM) mode, with programmed ionization modes witching and time segment scanning. The ion reactions for quantification were as follows: m/z 335.9��m/z 319.9(berberine, ESI+), m/z 639.1��m/z 160.9(plantamajoside, ESI-),m/z 779.3��m/z 617.4(saikosaponin a, ESI-),m/z 356.0��m/z 192.0(tetrahydropalmatine,ESI+),m/z 449.1��m/z 121.1(paeoniflorin, ESI-),m/z 456.1��m/z 323.1(amygdalin, ESI-),m/z 323.9��m/z 126.9(gliclazide, internal standard, ESI+) and m/z 321.9��m/z 170.1 (gliclazide, internal standard, ESI-). Blood samples were collected in heparinized tubes via the retinal venous plexus from each rat after a single oral dose of Jiawei Baiteng extracts(3.1 g��kg-1). The plasma samples were pretreated by methanol precipitation to remove protein components, and then analyzed by HPLC-TQ-MS/MS. The pharmacokinetic parameters of the bioactive components of Jiawei Baitengex tracts in rats were calculated by DAS (Drug and Statistics for Windows) software(Version 2.0). RESULTS The methodological test showed that the linear concentration ranges of berberine, plantamajoside, saikosaponin a, tetrahydropalmatine, paeoniflorin and amygdalin were 0.59-292.50, 0.68-168.75, 6.05-1 512.50, 0.68-337.50, 6.70-1 675.00 and 5.60-1 400.00 ng��mL-1, respectively. The limits of quantification (LOQs) of the six analytes were 0.59,0.68,6.05,0.68,6.70 and 5.60 ng��mL-1, respectively. The HPLC-TQ-MS/MS method was also validated with good precision, recovery and stability, which conformed to the analytical standards of biological samples. CONCLUSION The established method is proved to be sensitive, simple and reliable, which is suitable for the pharmacokinetic study of Jiawei Baiteng extracts.
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WU Jie, ZHU He, WU Jun, ZHONG Rong-ling, JU Jian-ming. Simultaneous Determination of Six Bioactive Components of Jiawei Baiteng Extracts in Plasma by HPLC-TQ-MS/MS and Their Pharmacokinetics in Rats. Chinese Pharmaceutical Journal, 2017, 52(6): 473-479.
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2017, Vol. 52 
