目的 探讨不同生态型太子参代谢物合成积累的差异。方法 采用1H-NMR代谢组学技术结合多元统计分析对野生与栽培太子参中化学成分进行比较分析,并找出差异成分。结果 共指认出35个化学成分;多元统计分析显示,野生与栽培太子参样品能明显分开,其中差异显著的化学成分有15个,其中丙氨酸、谷氨酸、琥珀酸、γ-氨基丁酸、乙酰乙酸、乳酸、亚油酸、苯丙氨酸、异亮氨酸、谷氨酰胺、赖氨酸、蔗糖的相对含量在野生太子参中较高;富马酸、棉子糖、木糖的相对含量在栽培太子参中较高。结论 本实验为揭示不同生态型太子参代谢物合成积累的变化规律及其药材的品质形成机制提供基础资料。
Abstract
OBJECTIVE To investigate the differences in the accumulation and biosynthesis of metabolites in different lactic acid of Pseudostellariae Radix. METHODS 1H-NMR based metabolomic approach combined with multivariate statistical analysis was used to investigate the chemical compositions and find the different metabolites in cultivated Pseudostellariae Radix and its wild-type. RESULTS Thirty-five metabolites were identified in the 1H-NMR spectra, and 15 of which showed remarkable differences. The multivariate statistical analysis showed that the cultivated Pseudostellariae Radix and its wild-type could be distinguished obviously. The contents of isoleucine, linolenic acid, lactic acid, alanine, lysine, glutamic acid, glutamine, acetoacetic acid, succinic acid, γ-aminobutyric acid, phenylalanine, and sucrose in cultivated Pseudostellaria heterophylla were lower than its wild-type, while the cultivated Pseudostellariae Radix contained more xylose, raffinose, and fumaric acid. CONCLUSION This study will provide basic information for exploring the quality formation mechanism and revealing the accumulation and biosynthesis of metabolites in different ecotypes of Pseudostellariae Radix.
关键词
太子参 /
野生 /
栽培 /
丙氨酸 /
谷氨酸 /
1H-NMR /
多元统计分析
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Key words
Pseudostellariae Radix /
wild type /
cultivated type /
alanine /
glutamic acid /
1H-NMR /
multivariate statistical analysis
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中图分类号：
R284
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参考文献
[1] Ch. P (2015) VolⅠ(中国药典2010版. 一部) [S]. 2015.
[2] FU X S, LIU X H, XU H, et al. Research status and trends of Pseudostellariae Radix [J]. Chin New Drugs J (中国新药杂志), 2012, 21(7):757-760.
[3] HIRSHI M, TAKASHI K, HIDEYULI K, et al. Pseudostellarins A-C, new tyrosinase inhibitory cyclic peptides from Pseudostellaria Heterophylla [J]. Tetrahedron, 1994, 50(23):6797-6804.
[4] HIRSHI M, TAKASHI K, HIDEYULI K, et al. Pseudostellarins D-F, new tyrosinase inhibitory cyclic peptides from Pseudostellaria Heterophylla [J]. Tetrahedron, 1994, 50(33):9975-9982.
[5] TAN N H, ZHOU J, CHEN C X, et al. Cyclopeptides from the roots of Pseudostellaria Heterophylla [J]. Phytochemistry, 1993, 32(5):1327-1330.
[6] AN K, HE J, WAN Z M, et al. Determination and multivariate statistical analysis of amino acid in Pseudostellaria Heterophylla from different producing areas [J]. Nat Prod Res Dev(天然产物研究与开发), 2012, 24(2):594-598.
[7] SONG J P, ZENG Y P, LIU X H, et al. Determination of polysaccharide in Radix Pseudostellariae from different habitats by HPLC-ELSD [J]. Shanghai J Tradit Chin Med(上海中医药杂志), 2008, 42(10):77-79.
[8] MA Y, HOU Y, ZOU L S, et al. Dynamic changes of nucleosides and nucleobases in Pseudostellariae Radix from Fujian province analyzed by QTRAP LC-MS/MS [J]. Chin J New Drugs(中国新药杂志), 2014, 23(19):2325-2330.
[9] FU X S, ZOU L S, LIU X H, et al. Analysis of cyclic peptides in Pseudostellariae Radix by UPLC-ESI-TOFMS/MS [J]. J Chin Mass Spec Soc (质谱学报), 2013, 34(3):179-184.
[10] GRIFFITHS L, IRVING A M. Assay by nuclear magnetic resonance spectroscopy:quantification limits [J]. Analyst, 1998, 123 (5):1061-1068.
[11] MI X, LI Z Y, QIN X M, et al. Monitoring of chemical components with different color traits of Tussilago farfara using NMR-based metabolomics [J]. Acta Pharm Sin(药学学报), 2013, 48(11):1692-1697.
[12] WANG X J, LI Z Y, XUE S Y, et al. Quality control over different processed products of Polygalae Radix based on plant metabolomics [J]. Chin Tradit Herb Drugs (中草药), 2012, 43(9):1727-1737.
[13] FAN G, TAO L H, YUE Q H, et al. Metabolic discrimination of Rhizoma Coptidis from different species using 1H-NMR spectroscopy and principal component analysis [J]. Planta Med, 2012, 78(6):641-648.
[14] TIAN D, LI Z Y, FAN S C, et al. NMR-based analysis of water soluble extracts of different Astragali Radix[J]. Acta Pharm Sin (药学学报), 2014, 49(1):89-94.
[15] SANDIPAN C, SHATAKSHII S, ASNA K, et al. Comprehensive metabolic fingerprinting of Withania somnifera leaf and root extracts [J]. Phytochemistry, 2010, 71(10):1085-1094.
[16] HYE K K, YOUNG H C, ROBERT V. NMR-based metabolomic analysis of plants [J]. Nat Protoc, 2010, 5(3):536-549.
[17] LI Z Y, FAN M L, QIN X M, et al. Comparison of chemical composition between raw and vinegar-baked Paeoniae Radix Alba using NMR based metabolomic approach [J]. Acta Pharm Sin (药学学报), 2015, 50(2):211-217.
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脚注
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基金
国家自然科学基金资助项目(81274016,81473312);江苏高校优势学科建设工程资助项目(ysxk-2014)
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