Abstract��OBJECTIVE To investigate extensively the cultural endophytic fungal community colonized mainly in the roots of five orchid medicinal plants species. METHODS Cultural endophytic fungi were identified using the morphological METHODS and molecular sequences. RESULTS A total of 241 strains of fungi were isolated and identified into at least 52 genera. Among them, Fusarium(14.9%), Colletotrichum(12.9%), Xylaria(5.4%) and Alternaria(3.7%) were dominated.Ten interested fungal strains were identified, including Dark septate endophyte (DSE) and Orchid mycorrhizal fungi(e.g. Tulasnella and Sebacina).CONCLUSION Fungal taxa and richness are related to host plant species and its geological distribution. This study provides important fungal resources for cultivation of orchid medicinal plants and screening of bioactive compounds in the future.
RASMUSSE H N, DIXON K W, JERSAKOVA J, et al. Germination and seedling establishment in orchids:a complex of requirements [J]. Ann Bot, 2015, 116(3):391-402.
[2]
GEBAUER G, PREISS K, GEBAUER A C. Partial mycoheterotrophy is more widespread among orchids than previously assumed [J]. New Phytol, 2016,211(1):11-15.
[3]
JACQUEMYN H, WAUD M, MERCKX V S, et al. Mycorrhizal diversity, seed germination and long-term changes in population size across nine populations of the terrestrial orchid Neottia ovate[J]. Mol Ecol, 2015,24(13):3269-3280.
[4]
WANG H, FANG H Y, WANG Y Q, et al. In situ seed baiting techniques in Dendrobium officinale Kimuraet Migo and Dendrobiumnobile Lindl. :the endangered Chinese endemic Dendrobium(Orchidaceae) [J]. World J Microbiol Biotechnol, 2011, 27(9):2051-2059.
[5]
KIRK P M, CANNON P F, MINTER D W,et al. Dictionary of The Fungi [M]. 10th ed. Oxon:CAB International, 2008.
[6]
WHITE T J, BRUNS T, LEE S, et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In:INNIS M A, GELFAND D H, SNINSKY J J. Whitd TJ (eds) PCR Protocols:A Guide to Methods and Applications [M]. Academic:Jenson Books Inc,1990.
JUMPPONEN A, TRAPPE J M. Dark septateendophytes:a review of facultative biotrophic root colonizing fungi [J]. New Phytologist, 1998, 140(2):295-310.
[9]
ADDY H D, PIERCEY M M, CURRAH R S. Microfungal endophytes in root [J]. Can J Bot, 2005, 83(1):1-13.
[10]
CHEN J, TAN X M, XING Y M, et al. Advances in research on diversity of endophytic and mycorhizal fungi of Dendrobium (Orchidaceae)[J]. Chin Pharm J(�й�ҩѧ��־), 2013,48(19):1649-1653.
[11]
K��RNER C. Functional Plant Ecology of High Mountain Ecosystems [M]. Beijing:Science Press, 2009.
[12]
WALLER F, ACHATZ B, BALTRUSCHAT H, et al. The endophytic fungus Piriformospora indica reprograms barley to salt-stress tolerance, disease resistance, and higher yield [J]. Proc Nat Acad Sci,2005, 102(38):13386-13391.
[13]
MARQUEZ L, REDMAN R, RODRIGUEZ R, et al. A virus in a fungus in a plant:three-way symbiosis required for thermal tolerance [J]. Science, 2007, 315(5811):513-515.
[14]
DIENE O, SAKAGAMI N, NARISAWA K. The role of dark septate endophytic fungal isolates in the accumulation of Cesium by Chinese cabbage and tomato plants under contaminated environments[J]. PLoS One, 2014, 9(10):e109233.
[15]
WANG J L, LI T, LIU G Y, et al. Unraveling the role of dark septate endophyte (DSE) colonizing maize (Zea mays) under cadmium stress:physiological, cytological and genic aspects [J]. Sci Rep, 2016, 6(1):22028.
[16]
WU L Q, LV Y L, MENG Z X, et al. The promoting role of an isolate of dark-septate fungus on its host plant Saussureainvolucrata Kar. et Kir [J]. Mycorrhiza, 2010, 20(2):127-135.